IF (Immunofluorescence)
(Immunofluorescence staining of ATP6AP2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ATP6AP2 polyclonal antibody (1:5000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Rabbit pro renin receptor/ATP6AP2 Polyclonal Antibody | anti-ATP6AP2 antibody
Anti-pro renin receptor/ATP6AP2 Antibody, Rabbit Polyclonal
Reactivity
Human, Mouse, Rat(Species predicted to react based on 100% sequence homology)
Purity
Protein A & Antigen Affinity
Synonyms
pro renin receptor/ATP6AP2, Antibody; Anti-pro renin receptor/ATP6AP2 Antibody, Rabbit Polyclonal; ATPase; H+ transporting; lysosomal accessory protein 2; Anti-APT6M8-9 Antibody; Anti-ATP6IP2 Antibody; Anti-ATP6M8-9 Antibody; Anti-ELDF10 Antibody; Anti-HT028 Antibody; Anti-M8-9 Antibody; Anti-MRXE Antibody; Anti-MRXSH Antibody; Anti-MSTP009 Antibody; Anti-PRR Antibody; Anti-RENR Antibody; Anti-XMRE Antibody; Anti-XPDS Antibody; anti-ATP6AP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
(Species predicted to react based on 100% sequence homology)
(Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human ATP6AP2/ATP6IP2
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ATP6AP2/ATP6IP2, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of ATP6AP2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ATP6AP2 polyclonal antibody (1:5000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of ATP6AP2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ATP6AP2 polyclonal antibody (1:5000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
WB (Western Blot)
(Anti-ATP6AP2 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:39 kDaObserved band size:34 kDa)
WB (Western Blot)
(Anti-ATP6AP2 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:39 kDaObserved band size:34 kDa)
Related Product Information for anti-ATP6AP2 antibody
ATP6AP2 (also known as the (pro)renin receptor) is a type I transmembrane protein that can be cleaved into two fragments in the Golgi apparatus. While in Drosophila ATP6AP2 functions in the planar cell polarity (PCP) pathway, recent human genetic studies have suggested that ATP6AP2 could participate in the assembly of the V-ATPase in the endoplasmic reticulum (ER). The (pro)renin receptor [(P)RR], also known as ATP6AP2 [ATPase 6 accessory protein 2], is highly expressed in the brain. ATP6AP2 plays a role in early brain development, adult hippocampal neurogenesis and in cognitive functions. Lack of ATP6AP2 has deleterious effects, and mutations of ATP6AP2 in humans are associated with, e.g. X-linked intellectual disability. The (pro)renin receptor [(P)RR, ATP6AP2] is a multifunctional transmembrane protein that activates local renin-angiotensin systems, but also interacts with Wnt pathways and vacuolar H(+) -ATPase (V-ATPase) during organogenesis. ATP6AP2 mutations in humans. Our results identify ATP6AP2 as an essential gene for the nervous system.
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Product Notes
The ATP6AP2 (Catalog #AAA258786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-pro renin receptor/ATP6AP2 Antibody, Rabbit Polyclonal reacts with Human, Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "pro renin receptor/ATP6AP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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