IP (Immunoprecipitation)
(Immunoprecipitating PHB in HEK293 whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA229103 in HEK293 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA229103 (8ug)+ HEK293 whole cell lysate (500ug)Lane 3: HEK293 whole cell lysate (10ug))
Rabbit anti-Human Prohibitin Polyclonal Antibody | anti-PHB antibody
Rabbit anti-human Prohibitin polyclonal Antibody
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Prohibitin, Antibody; Rabbit anti-human Prohibitin polyclonal Antibody; PHB; anti-PHB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
272
Applicable Applications for anti-PHB antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Prohibitin protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-18198 / sc-18196 / sc-28259
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IP (Immunoprecipitation)
(Immunoprecipitating PHB in HEK293 whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA229103 in HEK293 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA229103 (8ug)+ HEK293 whole cell lysate (500ug)Lane 3: HEK293 whole cell lysate (10ug))
IP (Immunoprecipitation)
(Immunoprecipitating PHB in HEK293 whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA229103 in HEK293 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA229103 (8ug)+ HEK293 whole cell lysate (500ug)Lane 3: HEK293 whole cell lysate (10ug))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA229103 at dilution of 1:10)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA229103 at dilution of 1:10)
WB (Western Blot)
(Western blotAll lanes: Prohibitin Antibody at 2ug/ml+mouse brain tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 30,18 kDaObserved band size: 30 kDa)
WB (Western Blot)
(Western blotAll lanes: Prohibitin Antibody at 2ug/ml+mouse brain tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 30,18 kDaObserved band size: 30 kDa)
Related Product Information for anti-PHB antibody
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3 untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3 untranslated region may function as a trans-acting regulatory RNA.
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3 untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3 untranslated region may function as a trans-acting regulatory RNA.
Product Categories/Family for anti-PHB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,018 Da
NCBI Official Full Name
prohibitin isoform 1
NCBI Official Synonym Full Names
prohibitin
NCBI Official Symbol
PHB
NCBI Official Synonym Symbols
PHB1; HEL-215; HEL-S-54e
NCBI Protein Information
prohibitin
UniProt Protein Name
Prohibitin
UniProt Gene Name
PHB
UniProt Entry Name
PHB_HUMAN
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Product Notes
The PHB phb (Catalog #AAA229103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Prohibitin polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Prohibitin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PHB phb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prohibitin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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