IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA229187 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human Proteasome subunit beta type-7 Polyclonal Antibody | anti-PSMB7 antibody
Rabbit anti-human Proteasome subunit beta type-7 polyclonal Antibody
Gene Names
PSMB7; Z
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Proteasome subunit beta type-7, Antibody; Rabbit anti-human Proteasome subunit beta type-7 polyclonal Antibody; Macropain chain Z Multicatalytic endopeptidase complex chain Z Proteasome subunit Z; anti-PSMB7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
277
Applicable Applications for anti-PSMB7 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Proteasome subunit beta type-7 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-54677 / sc-54735
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA229187 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells using AAA229187 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embeded human colorectal carcinoma using AAA229187 at dilution of 1:50)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embeded human colorectal carcinoma using AAA229187 at dilution of 1:50)
WB (Western Blot)
(Western blotAll lanes: Proteasome subunit beta type-7 antibody at 2ug/ml+293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 30,16 kDaObserved band size: 30.5 kDa)
WB (Western Blot)
(Western blotAll lanes: Proteasome subunit beta type-7 antibody at 2ug/ml+293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 30,16 kDaObserved band size: 30.5 kDa)
Related Product Information for anti-PSMB7 antibody
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,323 Da
NCBI Official Full Name
proteasome subunit beta type-7
NCBI Official Synonym Full Names
proteasome subunit beta 7
NCBI Official Symbol
PSMB7
NCBI Official Synonym Symbols
Z
NCBI Protein Information
proteasome subunit beta type-7
UniProt Protein Name
Proteasome subunit beta type-7
UniProt Gene Name
PSMB7
UniProt Synonym Gene Names
Z
UniProt Entry Name
PSB7_HUMAN
Similar Products
Product Notes
The PSMB7 psmb7 (Catalog #AAA229187) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Proteasome subunit beta type-7 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Proteasome subunit beta type-7 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PSMB7 psmb7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proteasome subunit beta type-7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.