<
IF (Immunofluorescence)
(Fluorescent confocal image of A2058 cell stained with PROX1 Antibody (C-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PROX1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). PROX1 immunoreactivity is localized to Nucleus significantly.)
Rabbit PROX1 Polyclonal Antibody | anti-PROX1 antibody
PROX1 Antibody (C-term)
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PROX1, Antibody; PROX1 Antibody (C-term); Prospero homeobox protein 1; Homeobox prospero-like protein PROX1; PROX-1; PROX1; anti-PROX1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PROX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-522 amino acids from the C-terminal region of human PROX1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 1.9 (varies by lot)
Sequence Positions
492-522
Sequence Length
737
Applicable Applications for anti-PROX1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of A2058 cell stained with PROX1 Antibody (C-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PROX1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). PROX1 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of A2058 cell stained with PROX1 Antibody (C-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PROX1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). PROX1 immunoreactivity is localized to Nucleus significantly.)
WB (Western Blot)
(PROX1 Antibody (K507) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PROX1 antibody detected the PROX1 protein (arrow).)
WB (Western Blot)
(PROX1 Antibody (K507) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PROX1 antibody detected the PROX1 protein (arrow).)
WB (Western Blot)
(Western blot analysis of PROX1 (arrow) using rabbit polyclonal PROX1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PROX1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of PROX1 (arrow) using rabbit polyclonal PROX1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PROX1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of lysates from A2058, rat PC-12 cell line (from left to right), using PROX1 Antibody (C-term). AAA284941 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A2058, rat PC-12 cell line (from left to right), using PROX1 Antibody (C-term). AAA284941 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from A2058 cell line, using PROX1 Antibody (C-term). AAA284941 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from A2058 cell line, using PROX1 Antibody (C-term). AAA284941 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Related Product Information for anti-PROX1 antibody
The expression pattern of the Prox1 homeo box gene suggests that it has a role in a variety of embryonic tissues, including lens. Analysis of mRNA reveals that Prox mRNA is present in many different human tissues and that lens demonstrated the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. The Prox1 gene is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been document to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.
Product Categories/Family for anti-PROX1 antibody
References
Nagai, H., et al., Genes Chromosomes Cancer 38(1):13-21 (2003).
Dyer, M.A., et al., Nat. Genet. 34(1):53-58 (2003).
Hong, Y.K., et al., Dev. Dyn. 225(3):351-357 (2002).
Petrova, T.V., et al., EMBO J. 21(17):4593-4599 (2002).
Mouta Carreira, C., et al., Cancer Res. 61(22):8079-8084 (2001).
Dyer, M.A., et al., Nat. Genet. 34(1):53-58 (2003).
Hong, Y.K., et al., Dev. Dyn. 225(3):351-357 (2002).
Petrova, T.V., et al., EMBO J. 21(17):4593-4599 (2002).
Mouta Carreira, C., et al., Cancer Res. 61(22):8079-8084 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83203
NCBI Official Full Name
prospero homeobox protein 1
NCBI Official Synonym Full Names
prospero homeobox 1
NCBI Official Symbol
PROX1
NCBI Protein Information
prospero homeobox protein 1
UniProt Protein Name
Prospero homeobox protein 1
UniProt Gene Name
PROX1
UniProt Synonym Gene Names
PROX-1
UniProt Entry Name
PROX1_HUMAN
Similar Products
Product Notes
The PROX1 prox1 (Catalog #AAA284941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 492-522. The PROX1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's PROX1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PROX1 prox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PROX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.