IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit PRP4 Polyclonal Antibody | anti-PRPF4B antibody
PRP4 Antibody (C-term)
Gene Names
PRPF4B; PR4H; PRP4; PRP4H; PRP4K; dJ1013A10.1
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRP4, Antibody; PRP4 Antibody (C-term); Serine/threonine-protein kinase PRP4 homolog; PRP4 kinase; PRP4 pre-mRNA-processing factor 4 homolog; PRPF4B; KIAA0536; PRP4; PRP4H; PRP4K; anti-PRPF4B antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PRP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 948-978 amino acids from the C-terminal region of human PRP4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
948-978
Sequence Length
1,007
Applicable Applications for anti-PRPF4B antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of anti-PRP4 Pab in Jurkat cell lysate. PRP4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of anti-PRP4 Pab in Jurkat cell lysate. PRP4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-PRPF4B antibody
Pre-mRNA splicing occurs in two sequential transesterification steps, and PRP4 is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).
References
Dellaire, G., et al., Mol. Cell. Biol. 22(14):5141-5156 (2002).
Kojima, T., et al., J. Biol. Chem. 276(34):32247-32256 (2001).
Huang, Y., et al., Biochem. Biophys. Res. Commun. 271(2):456-463 (2000).
Gross, T., et al., Nucleic Acids Res. 25(5):1028-1035 (1997).
Kojima, T., et al., J. Biol. Chem. 276(34):32247-32256 (2001).
Huang, Y., et al., Biochem. Biophys. Res. Commun. 271(2):456-463 (2000).
Gross, T., et al., Nucleic Acids Res. 25(5):1028-1035 (1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
116987
NCBI Official Full Name
serine/threonine-protein kinase PRP4 homolog
NCBI Official Synonym Full Names
pre-mRNA processing factor 4B
NCBI Official Symbol
PRPF4B
NCBI Official Synonym Symbols
PR4H; PRP4; PRP4H; PRP4K; dJ1013A10.1
NCBI Protein Information
serine/threonine-protein kinase PRP4 homolog
UniProt Protein Name
Serine/threonine-protein kinase PRP4 homolog
UniProt Gene Name
PRPF4B
UniProt Synonym Gene Names
KIAA0536; PRP4; PRP4H; PRP4K
UniProt Entry Name
PRP4B_HUMAN
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Product Notes
The PRPF4B prpf4b (Catalog #AAA290256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 948-978. The PRP4 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's PRP4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PRPF4B prpf4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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