WB (Western Blot)
(Dilution: Sample:DU145(Human) Cell Lysate at 30 ugPrimary: Anti-Prune homolog 2/C9orf65 (bs-19448R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 341 kDObserved band size: 341 kD)
Rabbit Prune homolog 2/C9orf65 Polyclonal Antibody | anti-C9orf65 antibody
Prune homolog 2/C9orf65 Polyclonal Antibody
Gene Names
PRUNE2; BMCC1; BNIPXL; C9orf65; KIAA0367; A214N16.3; bA214N16.3; RP11-58J3.2
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Prune homolog 2/C9orf65, Antibody; Prune homolog 2/C9orf65 Polyclonal Antibody; Protein prune homolog 2; BNIP2 motif-containing molecule at the C-terminal region 1; PRUNE2; BMCC1; BNIPXL; C9orf65; KIAA0367; anti-C9orf65 antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-C9orf65 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Dilution: Sample:DU145(Human) Cell Lysate at 30 ugPrimary: Anti-Prune homolog 2/C9orf65 (bs-19448R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 341 kDObserved band size: 341 kD)
WB (Western Blot)
(Dilution: Sample:DU145(Human) Cell Lysate at 30 ugPrimary: Anti-Prune homolog 2/C9orf65 (bs-19448R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 341 kDObserved band size: 341 kD)
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated (bs-19448R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated (bs-19448R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated (bs-19448R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C9orf65) Polyclonal Antibody, Unconjugated (bs-19448R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
340,635 Da
NCBI Official Full Name
protein prune homolog 2
NCBI Official Synonym Full Names
prune homolog 2 (Drosophila)
NCBI Official Symbol
PRUNE2
NCBI Official Synonym Symbols
BMCC1; BNIPXL; C9orf65; KIAA0367; A214N16.3; bA214N16.3; RP11-58J3.2
NCBI Protein Information
protein prune homolog 2; olfaxin; truncated PRUNE2; BNIP2 motif-containing molecule at the C-terminal region 1; BCH motif-containing molecule at the carboxyl terminal region 1; BNIP2 motif containing molecule at the carboxyl terminal region 1
UniProt Protein Name
Protein prune homolog 2
UniProt Gene Name
PRUNE2
UniProt Synonym Gene Names
BMCC1; BNIPXL; C9orf65; KIAA0367
UniProt Entry Name
PRUN2_HUMAN
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Product Notes
The C9orf65 prune2 (Catalog #AAA291841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Prune homolog 2/C9orf65 Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Prune homolog 2/C9orf65 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the C9orf65 prune2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prune homolog 2/C9orf65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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