IF (Immunofluorescence)
(AAA328707 staining A549 cells by IF/ICC. The samples werefixed with PFA and permeabilized in 0.1% Triton X-100,thenblocked in 10% serum for 45 minutes at 25°C. Samples werethen incubated with primary AAA328707 and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Rabbit PRUNE2 Polyclonal Antibody | anti-PRUNE2 antibody
PRUNE2 Antibody
Gene Names
PRUNE2; BMCC1; BNIPXL; C9orf65; KIAA0367
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Peptide Affinity Purification
Synonyms
PRUNE2, Antibody; PRUNE2 Antibody; A214N16 3; BCH motif containing molecule at the carboxyl terminal region 1; BMCC1; BNIP2 motif containing molecule at the carboxyl terminal region 1; BNIPXL; C9orf65; chromosome 9 open reading frame 65; prune homolog 2 (Drosophila); Prune2; anti-PRUNE2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
PRUNE2 Antibody detects endogenous levels of total PRUNE2.
Purity/Purification
Peptide Affinity Purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol.Store at -20°C
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PRUNE2 antibody
ELISA, IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human PRUNE2,corresponding to a region within N-terminal amino acids.
Preparation and Storage
Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328707 staining A549 cells by IF/ICC. The samples werefixed with PFA and permeabilized in 0.1% Triton X-100,thenblocked in 10% serum for 45 minutes at 25°C. Samples werethen incubated with primary AAA328707 and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(AAA328707 staining A549 cells by IF/ICC. The samples werefixed with PFA and permeabilized in 0.1% Triton X-100,thenblocked in 10% serum for 45 minutes at 25°C. Samples werethen incubated with primary AAA328707 and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Colo205, using PRUNE2Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Colo205, using PRUNE2Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PRUNE2 antibody
Description: May play an important role in regulating differentiation, survival and aggressiveness of the tumor cells.
Similarity: Belongs to the PPase class C family. Prune subfamily.
Similarity: Belongs to the PPase class C family. Prune subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
150 kDa
NCBI Official Full Name
protein prune homolog 2 isoform 1
NCBI Official Synonym Full Names
prune homolog 2 with BCH domain
NCBI Official Symbol
PRUNE2
NCBI Official Synonym Symbols
BMCC1; BNIPXL; C9orf65; KIAA0367
NCBI Protein Information
protein prune homolog 2
UniProt Protein Name
Protein prune homolog 2
UniProt Gene Name
PRUNE2
UniProt Synonym Gene Names
BMCC1; BNIPXL; C9orf65; KIAA0367
UniProt Entry Name
PRUN2_HUMAN
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Product Notes
The PRUNE2 prune2 (Catalog #AAA328707) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRUNE2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRUNE2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PRUNE2 prune2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRUNE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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