Rabbit PSA Polyclonal Antibody | anti-PSA antibody

PSA Antibody (Center)

Cat. Num. AAA293854

• Gene Names: NPEPPS; PSA; AAP-S; MP100
• Reactivity: Human; Predicted Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PSA, Antibody; PSA Antibody (Center); Puromycin-sensitive aminopeptidase; PSA; Cytosol alanyl aminopeptidase; AAP-S; NPEPPS; anti-PSA antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This PSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 536-565 amino acids from the Central region of human PSA.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

• Applicable Applications for anti-PSA antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
• Antigen Region: 536-565
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Dilution: IHC-P: 1:50~100PSA Antibody (Center) (Cat. #AAA293854) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Dilution: WB: 1:1000)

WB (Western Blot)

(Dilution: WB 1:2000All lanes : Anti-PSA Antibody (Center) at 1:2000 dilutionLane 1: T47D whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size : 110kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Dilution: WB 1:2000All lanes : Anti-PSA Antibody (Center) at 1:2000 dilutionLane 1: mouse brain tissue lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size : 110kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PSA antibody

PSA is the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.

Product Categories/Family for anti-PSA antibody

Primary Antibodies; Neuroscience

References

Thompson, M.W., Peptides 24 (9), 1359-1365 (2003)

NCBI and Uniprot Product Information

• NCBI GI #: 158937236
• NCBI GeneID: 9520
• NCBI Accession #: NP_006301.3
• NCBI GenBank Nucleotide #: NM_006310.3
• UniProt Accession #: P55786; Q11011
• Molecular Weight: 94,516 Da
• NCBI Official Full Name: puromycin-sensitive aminopeptidase
• NCBI Official Synonym Full Names: aminopeptidase puromycin sensitive
• NCBI Official Symbol: NPEPPS
• NCBI Official Synonym Symbols: PSA; AAP-S; MP100
• NCBI Protein Information: puromycin-sensitive aminopeptidase
• UniProt Protein Name: Puromycin-sensitive aminopeptidase
• UniProt Gene Name: NPEPPS
• UniProt Synonym Gene Names: PSA; PSA; AAP-S
• UniProt Entry Name: PSA_HUMAN