IP (Immunoprecipitation)
(Galectin3 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-Galectin3 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-Galectin3 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :30 kDa)
Rabbit anti-Human PSMA2 Polyclonal Antibody | anti-PSMA2 antibody
Anti-PSMA2 Antibody, Rabbit Polyclonal
Gene Names
PSMA2; MU; HC3; PSC2; PMSA2
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PSMA2, Antibody; Anti-PSMA2 Antibody, Rabbit Polyclonal; PSMA2 Antibody, Rabbit PAb, Antigen Affinity Purified; proteasome (prosome, macropain) subunit, alpha type, 2; Anti-HC3 Antibody; Anti-MU Antibody; Anti-PMSA2 Antibody; Anti-PSC2 Antibody; anti-PSMA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PSMA2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PSMA2 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the Human PSMA2
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PSMA2, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(Galectin3 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-Galectin3 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-Galectin3 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :30 kDa)
IP (Immunoprecipitation)
(Galectin3 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-Galectin3 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-Galectin3 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :30 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PSMA2 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PSMA2 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PSMA2 rabbit polyclonal antibody at 1:500 dilutionLane A: H1299 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)
WB (Western Blot)
(Anti-PSMA2 rabbit polyclonal antibody at 1:500 dilutionLane A: H1299 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)
Related Product Information for anti-PSMA2 antibody
PSMA2 (Proteasome 20S Subunit Alpha 2) is a Protein Coding gene. The PSMA2 gene, located on 7p14.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. The proteasome is a multi-catalytic proteinase complex with a highly ordered ring-shaped 20S core structure. This PSMA2 gene encodes a member of the peptidase T1A family, which is a 20S core alpha subunit. PSMA2 is widely expressed in the liver, fat, and other tissues. Diseases associated with PSMA2 include Cystic Fibrosis. The related pathways of PSMA2 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation. 233 organisms have orthologs with the human gene PSMA2.
References
Knuehl C, et al. (1996) Functional analysis of eukaryotic 20s proteasome nuclear localization signal. Exp Cell Res 225 (1): 67-74.Chen YX, et al. (2009) [expression of genes psma6 and slc25a4 in patients with acute monocytic leukemia]. Zhongguo Shi Yan Xue Ye Xue Za Zhi 17 (5): 1168-1173.Alvarez-Castelao B, et al. (2005) Mechanism of direct degradation of ikappabalpha by 20s proteasome. FEBS Lett 579 (21): 4797-4802.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
234
NCBI Official Full Name
Proteasome subunit alpha type-2
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, alpha type, 2
NCBI Official Symbol
PSMA2
NCBI Official Synonym Symbols
MU; HC3; PSC2; PMSA2
NCBI Protein Information
proteasome subunit alpha type-2; macropain subunit C3; proteasome subunit HC3; proteasome component C3; multicatalytic endopeptidase complex subunit C3
UniProt Protein Name
Proteasome subunit alpha type-2
UniProt Gene Name
PSMA2
UniProt Synonym Gene Names
HC3; PSC3
UniProt Entry Name
PSA2_HUMAN
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Product Notes
The PSMA2 psma2 (Catalog #AAA255188) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMA2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMA2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PSMA2 psma2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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