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IP (Immunoprecipitation)
(PSMB8 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate2 uL anti-PSMB8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMB8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size :30 kDa)
Rabbit anti-Human PSMB8 Polyclonal Antibody | anti-PSMB8 antibody
Anti-PSMB8 Antibody, Rabbit Polyclonal
Gene Names
PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PSMB8, Antibody; Anti-PSMB8 Antibody, Rabbit Polyclonal; PSMB8 Antibody, Rabbit PAb, Antigen Affinity Purified; proteasome (prosome, macropain) subunit, beta type, 8; Anti-ALDD Antibody; Anti-D6S216 Antibody; Anti-D6S216E Antibody; Anti-JMP Antibody; Anti-LMP7 Antibody; Anti-NKJO Antibody; Anti-PSMB5i Antibody; Anti-RING10 Antibody; anti-PSMB8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PSMB8
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PSMB8 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human PSMB8
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PSMB8, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PSMB8 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate2 uL anti-PSMB8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMB8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size :30 kDa)
IP (Immunoprecipitation)
(PSMB8 was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate2 uL anti-PSMB8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMB8 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size :30 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of PSMB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PSMB8 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of PSMB8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PSMB8 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PSMB8 in human lymphoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PSMB8 in human lymphoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PSMB8 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLane D: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:25 kDa)
WB (Western Blot)
(Anti-PSMB8 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLane D: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:25 kDa)
Related Product Information for anti-PSMB8 antibody
The proteasome subunit beta type 8 (PSMB8) gene encodes a catalytic subunit of immunoproteasome that plays a central role in the processing of antigenic peptides presented by major histocompatibility complex class I molecules. The proteasome subunit beta type 8 gene (PSMB8) encodes one of the beta subunits of the immunoproteasome responsible for the generation of peptides presented by major histocompatibility complex class I molecules. Dimorphic alleles of the PSMB8 gene, termed A and F types, based on the deduced 31st amino acid residue of the mature protein have been reported from various vertebrates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
276
NCBI Official Full Name
Proteasome subunit beta type-8
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, beta type, 8
NCBI Official Symbol
PSMB8
NCBI Official Synonym Symbols
JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
NCBI Protein Information
proteasome subunit beta type-8; macropain subunit C13; proteasome subunit Y2; protease component C13; proteasome component C13; proteasome-related gene 7; proteasome subunit beta 5i; low molecular mass protein 7; low molecular weight protein 7; proteasome
UniProt Protein Name
Proteasome subunit beta type-8
UniProt Gene Name
PSMB8
UniProt Synonym Gene Names
LMP7; PSMB5i; RING10; Y2
UniProt Entry Name
PSB8_HUMAN
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Product Notes
The PSMB8 psmb8 (Catalog #AAA254696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMB8 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMB8 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PSMB8 psmb8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMB8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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