Rabbit PSMD2 Polyclonal Antibody | anti-PSMD2 antibody

Anti-PSMD2 Antibody, Rabbit Polyclonal

Cat. Num. AAA255192

• Gene Names: PSMD2; S2; P97; Rpn1; TRAP2
• Reactivity: Human; Predicted to React with: Pongo abelii (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PSMD2, Antibody; Anti-PSMD2 Antibody, Rabbit Polyclonal; PSMD2 Antibody, Rabbit PAb, Antigen Affinity Purified; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; Anti-P97 Antibody; Anti-RPN1 Antibody; Anti-S2 Antibody; Anti-TRAP2 Antibody; anti-PSMD2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Pongo abelii (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSMD2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PSMD2 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human PSMD2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PSMD2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(PSMD2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-PSMD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMD2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size :100 kDa)

WB (Western Blot)

(Anti-PSMD2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:100 kDaObserved band size:100 kDa)

Related Product Information for anti-PSMD2 antibody

PSMD2 (Proteasome 26S Subunit, Non-ATPase 2) is a Protein Coding gene. The PSMD2 gene, located on 3q27.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signaling pathway since it interacts with the tumor necrosis factor type 1 receptor. PSMD2 is widely expressed in the testis, brain, and other tissues. Diseases associated with PSMD2 include Chapare Hemorrhagic Fever and Cystic Fibrosis. PSMD2 is a constituent of a signature associated with the acquisition of metastatic phenotype and poor prognosis in lung cancers. The related pathways of PSMD2 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation.

References

Tsurumi C, et al. (1996) Cdna cloning and functional analysis of the p97 subunit of the 26s proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11. Eur J Biochem 239 (3): 912-921.Tan Y, et al. (2019) Psmd1 and psmd2 regulate hepg2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism. BMC Mol Biol 20 (1): 24.Matsuyama Y, et al. (2011) Proteasomal non-catalytic subunit psmd2 as a potential therapeutic target in association with various clinicopathologic features in lung adenocarcinomas. Mol Carcinog 50 (4): 301-309.

NCBI and Uniprot Product Information

• NCBI GI #: 25777602
• NCBI GeneID: 5708
• NCBI Accession #: NP_002799.3
• NCBI GenBank Nucleotide #: NM_002808.3
• UniProt Accession #: Q13200; Q12932; Q15321; Q53XQ4; Q96I12
• Molecular Weight: 100,200 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 2
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
• NCBI Official Symbol: PSMD2
• NCBI Official Synonym Symbols: S2; P97; Rpn1; TRAP2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 2; 55.11 protein; protein 55.11; TNFR-associated protein 2; 26S proteasome subunit p97; 26S proteasome regulatory subunit S2; 26S proteasome regulatory subunit RPN1; tumor necrosis factor receptor-associated pr
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 2
• UniProt Gene Name: PSMD2
• UniProt Synonym Gene Names: TRAP2
• UniProt Entry Name: PSMD2_HUMAN