IP (Immunoprecipitation)
(PSME1 was immunoprecipitated using:; Lane A:0.5 mg 293T Whole Cell Lysate; Lane B:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-PSME1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-PSME1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 29 kDa; Observed band size :29 kDa)
Rabbit anti-Human PSME1 Polyclonal Antibody | anti-PSME1 antibody
PSME1 Antibody, Rabbit PAb, Antigen Affinity Purified
Gene Names
PSME1; PA28A; IFI5111; REGalpha; PA28alpha
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
PSME1, Antibody; PSME1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-IFI5111 Antibody; Anti-PA28A Antibody; Anti-PA28alpha Antibody; Anti-REGalpha Antibody; anti-PSME1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human Mouse, Rat, Pig (Species predicted to react based on 100% sequence homology)
Applicable Applications for anti-PSME1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PSME1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PSME1 was immunoprecipitated using:; Lane A:0.5 mg 293T Whole Cell Lysate; Lane B:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-PSME1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-PSME1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 29 kDa; Observed band size :29 kDa)
IP (Immunoprecipitation)
(PSME1 was immunoprecipitated using:; Lane A:0.5 mg 293T Whole Cell Lysate; Lane B:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-PSME1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-PSME1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 29 kDa; Observed band size :29 kDa)
WB (Western Blot)
(Anti-PSME1 rabbit monoclonal antibody at 1:500 dilution; Lane A: 293T Whole Cell Lysate; Lane B: HeLa Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:29 kDa; Observed band size:29 kDa)
WB (Western Blot)
(Anti-PSME1 rabbit monoclonal antibody at 1:500 dilution; Lane A: 293T Whole Cell Lysate; Lane B: HeLa Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:29 kDa; Observed band size:29 kDa)
Related Product Information for anti-PSME1 antibody
PA28A, also known as PSME1, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 2S core and a 19S regulator. The 2S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 1 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME1 gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring.
Product Categories/Family for anti-PSME1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,870 Da
NCBI Official Full Name
proteasome activator complex subunit 1 isoform 3
NCBI Official Synonym Full Names
proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
NCBI Official Symbol
PSME1
NCBI Official Synonym Symbols
PA28A; IFI5111; REGalpha; PA28alpha
NCBI Protein Information
proteasome activator complex subunit 1; 11S regulator complex subunit alpha; 29-kD MCP activator subunit; IGUP I-5111; activator of multicatalytic protease subunit 1; interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-
UniProt Protein Name
Proteasome activator complex subunit 1
UniProt Gene Name
PSME1
UniProt Synonym Gene Names
IFI5111; REG-alpha; IGUP I-5111; PA28a; PA28alpha
UniProt Entry Name
PSME1_HUMAN
Similar Products
Product Notes
The PSME1 psme1 (Catalog #AAA258618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSME1 Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSME1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PSME1 psme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSME1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.