IP (Immunoprecipitation)
(PSMG1 was immunoprecipitated using:Lane A:0.5 mg Jurkat U-251 MG Whole Cell Lysate4 uL anti-PSMG1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMG1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :27 kDa)
Rabbit anti-Human PSMG1 Polyclonal Antibody | anti-PSMG1 antibody
Anti-PSMG1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PSMG1, Antibody; Anti-PSMG1 Antibody, Rabbit Polyclonal; PSMG1 Antibody, Rabbit PAb, Antigen Affinity Purified; proteasome (prosome, macropain) assembly chaperone 1; Anti-C21LRP Antibody; Anti-DSCR2 Antibody; Anti-LRPC21 Antibody; Anti-PAC-1 Antibody; Anti-PAC1 Antibody; anti-PSMG1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PSMG1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PSMG1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
E Coli-derived Human PSMG1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PSMG1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PSMG1 was immunoprecipitated using:Lane A:0.5 mg Jurkat U-251 MG Whole Cell Lysate4 uL anti-PSMG1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMG1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :27 kDa)
IP (Immunoprecipitation)
(PSMG1 was immunoprecipitated using:Lane A:0.5 mg Jurkat U-251 MG Whole Cell Lysate4 uL anti-PSMG1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PSMG1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size :27 kDa)
WB (Western Blot)
(Anti-PSMG1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:28 kDa)
WB (Western Blot)
(Anti-PSMG1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:28 kDa)
Related Product Information for anti-PSMG1 antibody
PSMG1 (Proteasome Assembly Chaperone 1) is a Protein Coding gene. The PSMG1 gene, located on 21q22.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. PSMG1 is a chaperone protein that promotes the assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha-subunits into the heteroheptameric alpha ring, and prevents alpha ring dimerization. PSMG1 is widely expressed in the testis, thyroid, and other tissues. Diseases associated with PSMG1 include Down Syndrome and Thrombasthenia. 242 organisms have orthologs with the human gene PSMG1.
References
Wagner J, et al. (2013) Pseudomonas infection in children with early-onset crohn's disease: An association with a mutation close to psmg1. Inflamm Bowel Dis 19 (4): E58-59.Vidal-Taboada JM, et al. (1998) Identification and characterization of a new gene from human chromosome 21 between markers d21s343 and d21s268 encoding a leucine-rich protein. Biochem Biophys Res Commun 250 (3): 547-554.Vesa J, et al. (2005) Molecular and cellular characterization of the down syndrome critical region protein 2. Biochem Biophys Res Commun 328 (1): 235-242.
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Product Notes
The PSMG1 (Catalog #AAA256250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSMG1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSMG1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PSMG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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