Rabbit PTP1B Polyclonal Antibody | anti-PTP1B antibody

PTP1B (Ser-50), phospho-specific Antibody

Cat. Num. AAA71693

• Gene Names: PTPN1; PTP1B
• Reactivity: Human, Rat, Mouse
• Applications: Immunocytochemistry, ELISA, Western Blot
• Synonyms: PTP1B, Antibody; PTP1B (Ser-50), phospho-specific Antibody; PTP1B (Ser-50), phospho-specific; PTP1B Ser-50; anti-PTP1B antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified with phospho-PTP1B (Ser-50) peptide. The purified antibody detects a 50kDa* doublet corresponding to PTP1B in western blots of calyculin A treated human A431 and Jurkat cells. This reactivity is observed as a weak singlet in control cells and is not observed after lambda phosphatase treatment.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-PTP1B antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: PTP1B (Ser-50) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-50 in human PTP1B. This sequence has significant homology to the conserved site in rat and mouse PTP1B, and has homology to conserved sites in TC-PTP and MPTP, but not other protein tyrosine phosphatase family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of PTP1B in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-PTP1B (Ser-50) (PP2411) phosphospecific antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight 594.)

WB (Western Blot)

(Western blot image of human Jurkat cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with calyculin A (100nM for 30 min.). The blots were probed with mouse monoclonal anti-PTP1B (lanes 1 & 2) or rabbit polyclonal anti-PTP1B (Ser-50) (lanes 3 & 4).)

Related Product Information for anti-PTP1B antibody

PTP1B is a nonreceptor type protein tyrosine phosphatase that has essential roles in insulin and leptin signaling, as well as important functions in growth factor and integrin signaling. The structure of PTP1B includes a conserved phosphatase domain, C-terminal hydrophobic residues for targeting to the cytoplasmic face of the endoplasmic reticulum, and proline-rich regions characteristic of SH3 domain binding motifs. PTP1B can interact with N-Cadherin and dephosphorylate beta-catenin associated with cadherin complexes. PTP1B also interacts with Insulin and EGF receptors, and undergoes phosphorylation after receptor stimulation. Tyrosine phosphorylation at Tyr-66, Tyr-152, and Tyr-153 occurs after insulin receptor activation, and tyrosine phosphorylation of Tyr-152 may be required for interactions with N-Cadherin. In addition, Akt can phosphorylate Ser-50 and this phosphorylation can reduce PTP1B activity.

References

Lingamanaidu, V. et al. (2001) Molecular Endocrinology 15(10):1768.
Rhee, J. et al. (2001) J. Biol. Chem. 276(9):6640.
Bandyopadhyay, D. et al. (1997) J. Biol. Chem. 272(3):1639.

NCBI and Uniprot Product Information

• NCBI GI #: 4506289
• NCBI GeneID: 5770
• NCBI Accession #: NP_002818.1
• NCBI GenBank Nucleotide #: NM_002827.2
• UniProt Accession #: P18031; Q5TGD8; Q9BQV9; Q9NQQ4
• Molecular Weight: 49,967 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 1
• NCBI Official Symbol: PTPN1
• NCBI Official Synonym Symbols: PTP1B
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 1; protein tyrosine phosphatase 1B; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 1
• UniProt Gene Name: PTPN1
• UniProt Synonym Gene Names: PTP1B; PTP-1B
• UniProt Entry Name: PTN1_HUMAN