WB (Western Blot)
(Detection of human PTPN12 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTPN12 antibody AAA211404 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
Rabbit anti-Human PTPN12 Polyclonal Antibody | anti-PTPN12 antibody
Rabbit anti-PTPN12 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PTPN12, Antibody; Rabbit anti-PTPN12 Antibody, Affinity Purified; PTP-PEST; PTPG1; protein-tyrosine phosphatase G1; tyrosine-protein phosphatase non-receptor type 12; Tyrosine-protein phosphatase non-receptor type 12; protein tyrosine phosphatase, non-receptor type 12; anti-PTPN12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PTPN12 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 400 and 450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PTPN12 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTPN12 antibody AAA211404 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human PTPN12 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTPN12 antibody AAA211404 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PTPN12 by western blot of immunoprecipitation. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTPN12 antibody AAA211404 used for IP at 3 ug/mg lysate. PTPN12 was also immunoprecipitated by rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] . For blotting immunoprecipitated PTPN12, was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PTPN12 by western blot of immunoprecipitation. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTPN12 antibody AAA211404 used for IP at 3 ug/mg lysate. PTPN12 was also immunoprecipitated by rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] . For blotting immunoprecipitated PTPN12, was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 30 seconds.)
Related Product Information for anti-PTPN12 antibody
PTPN12 is a cytosolic protein tyrosine phosphatase (PTP) that contains a C-terminal PEST motif and plays an important role in cytoskeletal organization, immune cell activation, apoptosis, and embryonic development. PTPN12 appears to contribute to these cellular processes through its interaction with a variety of signaling and cytoskeletal-associated proteins. The recent discovery that PTPN12 is modulated by caspase-3 cleavage has led to the analysis of the role of PTPN12 and other PTPs as regulators of apoptosis.
Product Categories/Family for anti-PTPN12 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PTPN12 ptpn12 (Catalog #AAA211404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PTPN12 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PTPN12 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PTPN12 ptpn12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPN12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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