Rabbit PTPRN2 / Phogrin Polyclonal Antibody | anti-PTPRN2 antibody

Anti-PTPRN2 / Phogrin Antibody (aa206-255) IHC-plus

Cat. Num. AAA162217

• Gene Names: PTPRN2; IAR; ICAAR; PTPRP; IA-2beta; R-PTP-N2
• Reactivity: Mouse, Rat, Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Immunoaffinity purified
• Synonyms: PTPRN2 / Phogrin, Antibody; Anti-PTPRN2 / Phogrin Antibody (aa206-255) IHC-plus; Rabbit Polyclonal (IgG) to Human PTPRN2 / Phogrin; Human PTPRN2 / Phogrin; ICAAR; Ia-2 beta; IA-2beta; PTPRP; R-PTP-N2; Phogrin; Ptp-np; IAR; KIAA0387; PTP NE-6; Tyrosine phosphatase IA-2 beta; anti-PTPRN2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PTPRN2 Antibody detects endogenous levels of total PTPRN2 protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Sequence Length: 1015

• Applicable Applications for anti-PTPRN2 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen: PTPRN2 / Phogrin antibody was raised against synthetic peptide from human PTPRN2 (aa206-255).
• Immunogen Description: Synthetic peptide from human PTPRN2 (aa206-255).
• Epitope: aa206-255
• Target Species: Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation:; Formalin fixation and embedding in paraffin wax.; ; Tissue Sectioning:; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.; ; Deparaffinization:; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration:; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C for up to one year.

WB (Western Blot)

(Western blot of extracts from NIH-3T3 cells, using PTPRN2 Antibody. The lane on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry)

(Anti-PTPRN2 / Phogrin antibody IHC staining of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

NCBI and Uniprot Product Information

• NCBI GI #: 194097440
• NCBI GeneID: 5799
• NCBI Accession #: NP_002838.2
• NCBI GenBank Nucleotide #: NM_002847.4
• UniProt Accession #: Q92932; Q8N4I5; Q92662; Q9Y4F8; Q9Y4I6; E9PC57
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase N2 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type N2
• NCBI Official Symbol: PTPRN2
• NCBI Official Synonym Symbols: IAR; ICAAR; PTPRP; IA-2beta; R-PTP-N2
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase N2
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase N2
• UniProt Gene Name: PTPRN2
• UniProt Synonym Gene Names: KIAA0387; ICAAR
• UniProt Entry Name: PTPR2_HUMAN