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product-image-AAA210817_WB11.jpg WB (Western Blot) (Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody AAA210817 (lot AAA210817-3) used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

Goat anti-Human, Mouse Pumilio 1 Polyclonal Antibody | anti-PUM1 antibody

Goat anti-Pumilio 1 Antibody, Affinity Purified

Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Pumilio 1, Antibody; Goat anti-Pumilio 1 Antibody, Affinity Purified; pumilio homolog 1; SCA47; pumilio-1; PUMH1; PUMH; HSPUM; PUML1; Pumilio homolog 1; pumilio RNA binding family member 1; anti-PUM1 antibody
Ordering
Host
Goat
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PUM1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody AAA210817 (lot AAA210817-3) used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

product-image-AAA210817_WB11.jpg WB (Western Blot) (Detection of mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody AAA210817 (lot AAA210817-3) used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

WB (Western Blot)

(Detection of human Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody AAA210817 (lot AAA210817-3) used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

product-image-AAA210817_WB13.jpg WB (Western Blot) (Detection of human Pumillio 1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Pumillio 1 antibody AAA210817 (lot AAA210817-3) used for WB at 0.1 ug/ml. Secondary: HRP-conjugated rabbit anti-goat IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation)

(Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293Tcells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-Pumillio 1 antibody AAA210817 (lot AAA210817-3) used for IP at 6 ug per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210817-2) and rabbit anti-Pumillio 1 antibody For blotting immunoprecipitated Pumillio 1, AAA210817 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)

product-image-AAA210817_IP15.jpg IP (Immunoprecipitation) (Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293Tcells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-Pumillio 1 antibody AAA210817 (lot AAA210817-3) used for IP at 6 ug per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210817-2) and rabbit anti-Pumillio 1 antibody For blotting immunoprecipitated Pumillio 1, AAA210817 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-PUM1 antibody
Pumilio 1 is an evolutionarily conserved RNA binding protein that functions as a translational regulator of genes involved in embryogenesis and germline cell development. Pumilio 1, which is expressed in many tissue types, is homologous to the germline specific pumilio 2.
Product Categories/Family for anti-PUM1 antibody

NCBI and Uniprot Product Information

NCBI GeneID
UniProt Accession #
UniProt Protein Name
Pumilio homolog 1
UniProt Gene Name
PUM1
UniProt Synonym Gene Names
HsPUM; Pumilio-1

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Product Notes

The PUM1 pum1 (Catalog #AAA210817) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Pumilio 1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pumilio 1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PUM1 pum1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pumilio 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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