IP (Immunoprecipitation)
(PXN was immunoprecipitated using:; Lane A:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-PXN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-PXN rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 68 kDa; Observed band size :65 kDa)
Rabbit anti-Human PXN Polyclonal Antibody | anti-PXN antibody
PXN Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
PXN, Antibody; PXN Antibody, Rabbit PAb, Antigen Affinity Purified; anti-PXN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human PXN
Applicable Applications for anti-PXN antibody
IP (Immunoprecipitation), WB (Western Blot)
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PXN, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PXN was immunoprecipitated using:; Lane A:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-PXN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-PXN rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 68 kDa; Observed band size :65 kDa)
IP (Immunoprecipitation)
(PXN was immunoprecipitated using:; Lane A:0.5 mg HeLa Whole Cell Lysate; 4 uL anti-PXN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-PXN rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 68 kDa; Observed band size :65 kDa)
WB (Western Blot)
(Anti-PXN rabbit polyclonal antibody at 1:500 dilution; Lane A: 293T Whole Cell Lysate; Lane B: HeLa Whole Cell Lysate; Lane C: MCF7 Whole Cell Lysate; Lane D: Jurkat Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:68 kDa; Observed band size:68 kDa)
WB (Western Blot)
(Anti-PXN rabbit polyclonal antibody at 1:500 dilution; Lane A: 293T Whole Cell Lysate; Lane B: HeLa Whole Cell Lysate; Lane C: MCF7 Whole Cell Lysate; Lane D: Jurkat Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:68 kDa; Observed band size:68 kDa)
Related Product Information for anti-PXN antibody
PXN was significantly upregulated in cervical cancer, which associated with tumor stage, poor differentiation, lymphovascular space invasion and lymphatic metastasis. Knockdown of PXN notably impaired cellular growth and colony formation by suppressing Bcl-2 and inducing marked apoptosis. Moreover, PXN led to resistance to radiation, and downregulation of PXN resensitized C33A cells to radiation.PXN was frequently upregulated and acted as an oncogene via regulating Bcl-2 in cervical cancer, which supports PXN as a potent therapeutic target.
Product Categories/Family for anti-PXN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,505 Da
NCBI Official Full Name
paxillin isoform 2
NCBI Official Synonym Full Names
paxillin
NCBI Official Symbol
PXN
NCBI Protein Information
paxillin
UniProt Protein Name
Paxillin
UniProt Gene Name
PXN
UniProt Entry Name
PAXI_HUMAN
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Product Notes
The PXN pxn (Catalog #AAA258623) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PXN Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PXN can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PXN pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PXN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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