WB (Western Blot)
(Detection of human and mouse PYGL by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody AAA212643 (lot AAA212643-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse PYGL Polyclonal Antibody | anti-PYGL antibody
Rabbit anti-PYGL Antibody, Affinity Purified
Gene Names
PYGL; GSD6
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PYGL, Antibody; Rabbit anti-PYGL Antibody, Affinity Purified; glycogen phosphorylase, liver form; GSD6; phosphorylase, glycogen, liver; Glycogen phosphorylase, liver form; glycogen phosphorylase L; anti-PYGL antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PYGL antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 797 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PYGL by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody AAA212643 (lot AAA212643-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse PYGL by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody AAA212643 (lot AAA212643-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human PYGL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from 293T cells. Antibodies: Affinity purified rabbit anti-PYGL antibody AAA212643 (lot AAA212643-1) used for IP at 6 ug per reaction. PYGL was also immunoprecipitated by rabbit anti-PYGL antibody BL15846. For blotting immunoprecipitated PYGL, AAA212643 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PYGL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from 293T cells. Antibodies: Affinity purified rabbit anti-PYGL antibody AAA212643 (lot AAA212643-1) used for IP at 6 ug per reaction. PYGL was also immunoprecipitated by rabbit anti-PYGL antibody BL15846. For blotting immunoprecipitated PYGL, AAA212643 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-PYGL antibody
Glycogen phosphorylase, liver form, (PYGL) encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues [taken from NCBI Entrez Gene (Gene ID: 5836)].
Product Categories/Family for anti-PYGL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,134 Da
NCBI Official Full Name
glycogen phosphorylase, liver form isoform 1
NCBI Official Synonym Full Names
phosphorylase, glycogen, liver
NCBI Official Symbol
PYGL
NCBI Official Synonym Symbols
GSD6
NCBI Protein Information
glycogen phosphorylase, liver form
UniProt Protein Name
Glycogen phosphorylase, liver form
UniProt Gene Name
PYGL
UniProt Entry Name
PYGL_HUMAN
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Product Notes
The PYGL pygl (Catalog #AAA212643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PYGL Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PYGL can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PYGL pygl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PYGL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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