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IHC (Immunohistochemistry)
(Pyruvate Kinase (PKM2) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit Pyruvate Kinase (PKM2) Polyclonal Antibody | anti-PKM antibody
Pyruvate Kinase (PKM2) Antibody (C-term)
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human, mouse, rat, monkey
Applications
Immunohistochemistry, Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Pyruvate Kinase (PKM2), Antibody; Pyruvate Kinase (PKM2) Antibody (C-term); Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58; PKM; OIP3; PK2; PK3; PKM2; anti-PKM antibody
Host
Rabbit
Reactivity
Human, mouse, rat, monkey
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
476-505
Sequence Length
605
Applicable Applications for anti-PKM antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Pyruvate Kinase (PKM2) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Pyruvate Kinase (PKM2) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-h PKM2-N491 Pab in Ramos cell line lysates (35ug/lane). hPKM2-N491(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-h PKM2-N491 Pab in Ramos cell line lysates (35ug/lane). hPKM2-N491(arrow) was detected using the purified Pab.)
IF (Immunofluorescence)
(Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA286433 Pyruvate Kinase (PKM2) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).)
IF (Immunofluorescence)
(Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA286433 Pyruvate Kinase (PKM2) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).)
WB (Western Blot)
(Western blot analysis of lysates from HeLa, rat PC-12, Ramos, mouse NIH/3T3 cell line, mouse brain tissue lysate(from left to right), using PKM2-N491. AAA286433 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from HeLa, rat PC-12, Ramos, mouse NIH/3T3 cell line, mouse brain tissue lysate(from left to right), using PKM2-N491. AAA286433 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from HeLa, rat PC-12, Ramos, mouse NIH/3T3 cell line, mouse brain tissue lysate(from left to right), using PKM2-N491. AAA286433 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from HeLa, rat PC-12, Ramos, mouse NIH/3T3 cell line, mouse brain tissue lysate(from left to right), using PKM2-N491. AAA286433 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Related Product Information for anti-PKM antibody
There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
Product Categories/Family for anti-PKM antibody
References
References for protein:
1.Williams, J.M., et al., Mol. Microbiol. 27(1):171-186 (1998).
2.Gress, T.M., et al., Oncogene 13(8):1819-1830 (1996).
3.Kato, H., et al., Proc. Natl. Acad. Sci. U.S.A. 86(20):7861-7865 (1989).
4.Tsutsumi, H., et al., Genomics 2(1):86-89 (1988).
5.Tani, K., et al., Gene 73(2):509-516 (1988).
References for MCF7 cell line:
1.Soule, HD; Vazquez J; Long A; Albert S; Brennan M. (1973). "A human cell line from a pleural effusion derived from a breast carcinoma". Journal of the National Cancer Institute 51 (5): 1409-1416. [PMID 4357757].
2.Levenson, AS; Jordan VC. (1997). "MCF-7: the first hormone-responsive breast cancer cell line". Cancer Research 57 (15): 3071-3078. [PMID 9242427].
3.Lacroix, M; Leclercq G. (2004). "Relevance of breast cancer cell lines as models for breast tumours: an update". Breast Research and Treatment 83 (3): 249-289.[PMID 14758095].
1.Williams, J.M., et al., Mol. Microbiol. 27(1):171-186 (1998).
2.Gress, T.M., et al., Oncogene 13(8):1819-1830 (1996).
3.Kato, H., et al., Proc. Natl. Acad. Sci. U.S.A. 86(20):7861-7865 (1989).
4.Tsutsumi, H., et al., Genomics 2(1):86-89 (1988).
5.Tani, K., et al., Gene 73(2):509-516 (1988).
References for MCF7 cell line:
1.Soule, HD; Vazquez J; Long A; Albert S; Brennan M. (1973). "A human cell line from a pleural effusion derived from a breast carcinoma". Journal of the National Cancer Institute 51 (5): 1409-1416. [PMID 4357757].
2.Levenson, AS; Jordan VC. (1997). "MCF-7: the first hormone-responsive breast cancer cell line". Cancer Research 57 (15): 3071-3078. [PMID 9242427].
3.Lacroix, M; Leclercq G. (2004). "Relevance of breast cancer cell lines as models for breast tumours: an update". Breast Research and Treatment 83 (3): 249-289.[PMID 14758095].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57937
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
NCBI Protein Information
pyruvate kinase PKM
UniProt Protein Name
Pyruvate kinase PKM
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN
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Product Notes
The PKM pkm (Catalog #AAA286433) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 476-505. The Pyruvate Kinase (PKM2) Antibody (C-term) reacts with Human, mouse, rat, monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Pyruvate Kinase (PKM2) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PKM pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Kinase (PKM2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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