IP (Immunoprecipitation)
(RAB11B was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg U87-MG Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell LysateLane D:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-RAB11B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RAB11B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :24 kDa)
Rabbit anti-Human RAB11B Polyclonal Antibody | anti-RAB11B antibody
Anti-RAB11B Antibody, Rabbit Polyclonal
IP (Immunoprecipitation)
(RAB11B was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg U87-MG Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell LysateLane D:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-RAB11B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RAB11B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :24 kDa)
IP (Immunoprecipitation)
(RAB11B was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg U87-MG Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell LysateLane D:0.5 mg SH-SY5Y Whole Cell Lysate4 uL anti-RAB11B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RAB11B rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :24 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of S1h-3C-RAB11B in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human S1h-3C-RAB11B polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of S1h-3C-RAB11B in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human S1h-3C-RAB11B polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
WB (Western Blot)
(Anti-RAB11B rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: U87-MG Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: SH-SY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa)
WB (Western Blot)
(Anti-RAB11B rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: U87-MG Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: SH-SY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa)
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Product Notes
The RAB11B (Catalog #AAA258928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAB11B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB11B can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RAB11B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB11B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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