IF (Immunofluorescence)
(Immunofluorescent analysis of RAB31 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit RAB31 Polyclonal Antibody | anti-RAB31 antibody
Anti-RAB31 Antibody
Gene Names
RAB31; Rab22B
Reactivity
Human, Mouse, Rat, Bovine, Pig
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
RAB31, Antibody; Anti-RAB31 Antibody; RAB22B; Ras-related protein Rab-31; Ras-related protein Rab-22B; anti-RAB31 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of RAB31 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
195
Applicable Applications for anti-RAB31 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAB31. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of RAB31 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of RAB31 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Western blot analysis of RAB31 expression in MCF7 (A), Raw264.7 (B), rat brain (C) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of RAB31 expression in MCF7 (A), Raw264.7 (B), rat brain (C) whole cell lysates.)
Related Product Information for anti-RAB31 antibody
Rabbit polyclonal antibody to RAB31
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,569 Da
NCBI Official Full Name
ras-related protein Rab-31
NCBI Official Synonym Full Names
RAB31, member RAS oncogene family
NCBI Official Symbol
RAB31
NCBI Official Synonym Symbols
Rab22B
NCBI Protein Information
ras-related protein Rab-31; ras-related protein Rab-22B
UniProt Protein Name
Ras-related protein Rab-31
UniProt Gene Name
RAB31
UniProt Synonym Gene Names
RAB22B
UniProt Entry Name
RAB31_HUMAN
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Product Notes
The RAB31 rab31 (Catalog #AAA104880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAB31 Antibody reacts with Human, Mouse, Rat, Bovine, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's RAB31 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the RAB31 rab31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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