WB (Western Blot)
(Western blot analysis of extracts from COS cells, using RAB3GAP2 antibody.)
Rabbit RAB3GAP2 Polyclonal Antibody | anti-RAB3GAP2 antibody
RAB3GAP2 Antibody
Gene Names
RAB3GAP2; p150; SPG69; WARBM2; RAB3GAP150; RAB3-GAP150
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RAB3GAP2, Antibody; RAB3GAP2 Antibody; DKFZp434D245; FLJ14579; KIAA0839; p150; Rab3 GAP p150; Rab3 GAP regulatory subunit; Rab3 GAP150; Rab3 GTPase activating protein 150 kDa subunit; Rab3 GTPase activating protein non catalytic subunit; RAB3 GTPase activating protein subunit 2 (non catalytic); RAB3 GTPase activating protein subunit 2; RAB3GAP150; RGAP iso; RP11 568G11.1; anti-RAB3GAP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RAB3GAP2 antibody detects endogenous levels of total RAB3GAP2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1393
Applicable Applications for anti-RAB3GAP2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasmic. Note: In neurons, it is enriched in the synaptic soluble fraction.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (92%), Bovine (92%), Sheep (92%), Rabbit (100%), Dog (92%), Chicken (92%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from COS cells, using RAB3GAP2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from COS cells, using RAB3GAP2 antibody.)
IHC (Immunohiostchemistry)
(AAA325026 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA325026 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence)
(AAA325026 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA325026 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-RAB3GAP2 antibody
Function: Regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.
Subunit Structure: The Rab3 GTPase-activating complex is a heterodimer composed of RAB3GAP and RAB3-GAP150. The Rab3 GTPase-activating complex interacts with DMXL2 (By similarity). Interacts with LMAN1 (PubMed:22337587).
Similarity: Belongs to the Rab3-GAP regulatory subunit family.
Subunit Structure: The Rab3 GTPase-activating complex is a heterodimer composed of RAB3GAP and RAB3-GAP150. The Rab3 GTPase-activating complex interacts with DMXL2 (By similarity). Interacts with LMAN1 (PubMed:22337587).
Similarity: Belongs to the Rab3-GAP regulatory subunit family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 156 kD
Predicted: 156 kDa
Predicted: 156 kDa
NCBI Official Full Name
rab3 GTPase-activating protein non-catalytic subunit
NCBI Official Synonym Full Names
RAB3 GTPase activating non-catalytic protein subunit 2
NCBI Official Symbol
RAB3GAP2
NCBI Official Synonym Symbols
p150; SPG69; WARBM2; RAB3GAP150; RAB3-GAP150
NCBI Protein Information
rab3 GTPase-activating protein non-catalytic subunit
UniProt Protein Name
Rab3 GTPase-activating protein non-catalytic subunit
UniProt Gene Name
RAB3GAP2
UniProt Synonym Gene Names
KIAA0839; Rab3-GAP150
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Product Notes
The RAB3GAP2 rab3gap2 (Catalog #AAA325026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB3GAP2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB3GAP2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RAB3GAP2 rab3gap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB3GAP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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