Rabbit Rab5 Polyclonal Antibody | anti-RAB5A antibody

Rab5 Antibody: ATTO 390

Cat. Num. AAA103495

• Gene Names: RAB5A; RAB5
• Reactivity: Human, Mouse, Monkey, Bovine, Rat
• Applications: Immunofluorescence, Western Blot
• Synonyms: Rab5, Antibody; Rab5 Antibody: ATTO 390; Rab 5A; RAS associated protein RAB5A; Ras related protein Rab 5 A; anti-RAB5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Monkey, Bovine, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-RAB5A antibody: IF (Immunofluorescence), WB (Western Blot)
• Conjugate: ATTO 390
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

IHC (Immunohiostchemistry)

(Immunohistochemistry analysis using Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)

WB (Western Blot)

(Western blot analysis of Human Cell line lysates showing detection of Rab5 protein using Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (SPC-168) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Related Product Information for anti-RAB5A antibody

Background Info: The antibody recognizes 26kDa Rab5 of the above species origins.

Scientific Background: Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.

Product Categories/Family for anti-RAB5A antibody

Cell Signaling; Neuroscience; Organelle Markers

References

1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.
1. Ballmer-Hofer, K., Andersson, A.E., Ratcliffe, L.E. and Berger, P. (2011). Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output. Blood. 118 (3), 816-826. doi: 10.1182/blood-2011-01-328773.

NCBI and Uniprot Product Information

• NCBI GI #: 19923262
• NCBI GeneID: 5868
• NCBI Accession #: NP_004153.2
• NCBI GenBank Nucleotide #: NM_004162.4
• UniProt Accession #: Q6FI44; B4DJA5; P20339
• Molecular Weight: 22,178 Da
• NCBI Official Full Name: ras-related protein Rab-5A isoform 1
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A; RAS-associated protein RAB5A
• UniProt Protein Name: Ras-related protein Rab-5A
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5
• UniProt Entry Name: RAB5A_HUMAN