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IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using RAB7A antibody (A12784) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Rabbit RAB7A Polyclonal Antibody | anti-RAB7A antibody
RAB7A Polyclonal Antibody
Gene Names
RAB7A; RAB7; PRO2706
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Affinity Purification
Synonyms
RAB7A, Antibody; RAB7A Polyclonal Antibody; PRO2706; RAB7; anti-RAB7A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
207
Applicable Applications for anti-RAB7A antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RAB7A (NP_004628.4)
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using RAB7A antibody (A12784) at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using RAB7A antibody (A12784) at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using RAB7A antibody (AAA28256) at dilution of 1:100. Blue: DAPI for nuclearstaining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using RAB7A antibody (AAA28256) at dilution of 1:100. Blue: DAPI for nuclearstaining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using RAB7A antibody (AAA28256) at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using RAB7A antibody (AAA28256) at dilution of 1:100. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using RAB7A antibody (AAA28256) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Enhanced Kit (RM00021)._Exposure time: 90s)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using RAB7A antibody (AAA28256) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Enhanced Kit (RM00021)._Exposure time: 90s)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using RAB7A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using RAB7A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Related Product Information for anti-RAB7A antibody
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.
Product Categories/Family for anti-RAB7A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 23 kDa
Calculated MW: 23 kDa
Calculated MW: 23 kDa
NCBI Official Full Name
Ras-related protein Rab-7a
NCBI Official Synonym Full Names
RAB7A, member RAS oncogene family
NCBI Official Symbol
RAB7A
NCBI Official Synonym Symbols
RAB7; PRO2706
NCBI Protein Information
ras-related protein Rab-7a
UniProt Protein Name
Ras-related protein Rab-7a
UniProt Gene Name
RAB7A
UniProt Synonym Gene Names
RAB7
Similar Products
Product Notes
The RAB7A rab7a (Catalog #AAA28256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB7A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB7A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the RAB7A rab7a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB7A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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