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FCM/FACS (Flow Cytometry)
(RACGAP1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human RACGAP1 Polyclonal Antibody | anti-RACGAP1 antibody
RACGAP1 Antibody (N-term)
Gene Names
RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RACGAP1, Antibody; RACGAP1 Antibody (N-term); Rac GTPase-activating protein 1; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolog; CYK4; HsCYK-4; RACGAP1 HGNC:9804); anti-RACGAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RACGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human RACGAP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.26 (varies by lot)
Sequence Positions
23-50
Sequence Length
632
Applicable Applications for anti-RACGAP1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(RACGAP1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(RACGAP1 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(RACGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RACGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(RACGAP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RACGAP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(RACGAP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow).)
WB (Western Blot)
(RACGAP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow).)
Related Product Information for anti-RACGAP1 antibody
Rho GTPases control a variety of cellular processes. There
are 3 subtypes of Rho GTPases in the Ras superfamily of small G
proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and
CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind
activated forms of Rho GTPases and stimulate GTP hydrolysis.
Through this catalytic function, Rho GAPs negatively regulate
Rho-mediated signals. GAPs may also serve as effector molecules and
play a role in signaling downstream of Rho and other Ras-like
GTPases.
are 3 subtypes of Rho GTPases in the Ras superfamily of small G
proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and
CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind
activated forms of Rho GTPases and stimulate GTP hydrolysis.
Through this catalytic function, Rho GAPs negatively regulate
Rho-mediated signals. GAPs may also serve as effector molecules and
play a role in signaling downstream of Rho and other Ras-like
GTPases.
Product Categories/Family for anti-RACGAP1 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009)
Simon, G.C., et al. EMBO J. 27(13):1791-1803(2008)
Toure, A., et al. FEBS Lett. 582(8):1182-1188(2008)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009)
Simon, G.C., et al. EMBO J. 27(13):1791-1803(2008)
Toure, A., et al. FEBS Lett. 582(8):1182-1188(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71027
NCBI Official Full Name
rac GTPase-activating protein 1
NCBI Official Synonym Full Names
Rac GTPase activating protein 1
NCBI Official Symbol
RACGAP1
NCBI Official Synonym Symbols
CYK4; ID-GAP; HsCYK-4; MgcRacGAP
NCBI Protein Information
rac GTPase-activating protein 1
UniProt Protein Name
Rac GTPase-activating protein 1
UniProt Gene Name
RACGAP1
UniProt Synonym Gene Names
MgcRacGAP; CYK4; HsCYK-4
UniProt Entry Name
RGAP1_HUMAN
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Product Notes
The RACGAP1 racgap1 (Catalog #AAA283698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 23-50. The RACGAP1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RACGAP1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RACGAP1 racgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RACGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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