WB (Western Blot)
(Anti- RACK1 antibody, AAA46363, Western blottingAll lanes: Anti RACK1 (AAA46363) at 0.5ug/mlLane 1: Mouse Liver Tissue Lysate at 50ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: Mouse Spleen Tissue Lysate at 50ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugLane 6: HEPA Whole Cell Lysate at 40ugLane 7: RH35 Whole Cell Lysate at 40ugPredicted bind size: 35KDObserved bind size: 35KD)
RACK1 Polyclonal Antibody | anti-RACK1 antibody
Anti-RACK1 Antibody
Gene Names
RACK1; H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
RACK1, Antibody; Anti-RACK1 Antibody; Guanine nucleotide-binding protein subunit beta-2-like 1; Cell proliferation-inducing gene 21 protein; GBLP_HUMAN; Gnb2 rs1; Gnb2-rs1; GNB2L1; Gnb2rs1; Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1; Guanine nucleotide binding protein beta polypeptide 2 like 1; Guanine nucleotide binding protein beta subunit 2 like 1; Guanine nucleotide binding protein beta subunit like protein 12.3; Guanine nucleotide binding protein subunit beta 2 like 1; Guanine nucleotide binding protein subunit beta like protein 12.3 antibodyGuanine nucleotide-binding protein subunit beta-2-like 1; Guanine nucleotide-binding protein subunit beta-like protein 12.3; H12.3; HLC 7; HLC-7; HLC7; Human lung cancer oncogene 7 protein; Lung cancer oncogene 7; OTTHUMP00000223704; OTTHUMP00000223870; OTTHUMP00000223891; OTTHUMP00000223893; OTTHUMP00000223900; OTTHUMP00000223902; OTTHUMP00000223930; OTTHUMP00000223931; PIG 21; PIG21; Proliferation inducing gene 21; Protein homologous to chicken B complex protein guanine nucleotide binding; RACK 1; RACK1; Receptor for activated C kinase; Receptor for activated C kinase 1; Receptor of activated protein kinase C 1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; anti-RACK1 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
317
Applicable Applications for anti-RACK1 antibody
WB (Western Blot)
Immunogen
E Coli-derived human RACK1 recombinant protein (Position: T2-R317). Human RACK1 shares 100% amino acid (aa) sequence identity with both mouse and rat RACK1.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti- RACK1 antibody, AAA46363, Western blottingAll lanes: Anti RACK1 (AAA46363) at 0.5ug/mlLane 1: Mouse Liver Tissue Lysate at 50ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: Mouse Spleen Tissue Lysate at 50ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugLane 6: HEPA Whole Cell Lysate at 40ugLane 7: RH35 Whole Cell Lysate at 40ugPredicted bind size: 35KDObserved bind size: 35KD)
WB (Western Blot)
(Anti- RACK1 antibody, AAA46363, Western blottingAll lanes: Anti RACK1 (AAA46363) at 0.5ug/mlLane 1: Mouse Liver Tissue Lysate at 50ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: Mouse Spleen Tissue Lysate at 50ugLane 4: SMMC Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugLane 6: HEPA Whole Cell Lysate at 40ugLane 7: RH35 Whole Cell Lysate at 40ugPredicted bind size: 35KDObserved bind size: 35KD)
WB (Western Blot)
(Anti- RACK1 antibody, AAA46363, Western blottingAll lanes: Anti RACK1 (AAA46363) at 0.5ug/mlWB: Recombinant Human RACK1 Protein 0.5ngPredicted bind size: 35KDObserved bind size: 35KD)
WB (Western Blot)
(Anti- RACK1 antibody, AAA46363, Western blottingAll lanes: Anti RACK1 (AAA46363) at 0.5ug/mlWB: Recombinant Human RACK1 Protein 0.5ngPredicted bind size: 35KDObserved bind size: 35KD)
Related Product Information for anti-RACK1 antibody
Description: Rabbit IgG polyclonal antibody for Guanine nucleotide-binding protein subunit beta-2-like 1(GNB2L1) detection. Tested with WB in Human;Mouse;Rat.
Background: Guanine nucleotide-binding protein subunit beta-2-like 1, also known as RACK1 is a 32kDa protein that in humans is encoded by the GNB2L1 gene. It is mapped to 5q35.3. GNB2L1 is a major component of translating ribosomes, which harbor significant amounts of PKC. This gene can provide a physical and functional link between PKC signaling and ribosome activation. GNB2L1 is also a mediator of agonist-induced Ca(2+) release. What's more, GNB2L1 is an essential component of an oxygen-independent mechanism for regulating HIF1A stability. Overexpression of GNB2L1 and PKC-alpha suppressed CLOCK-BMAL1 transcriptional activity, and GNB2L1 stimulated phosphorylation of BMAL1 by PKC-alpha in vitro.
Background: Guanine nucleotide-binding protein subunit beta-2-like 1, also known as RACK1 is a 32kDa protein that in humans is encoded by the GNB2L1 gene. It is mapped to 5q35.3. GNB2L1 is a major component of translating ribosomes, which harbor significant amounts of PKC. This gene can provide a physical and functional link between PKC signaling and ribosome activation. GNB2L1 is also a mediator of agonist-induced Ca(2+) release. What's more, GNB2L1 is an essential component of an oxygen-independent mechanism for regulating HIF1A stability. Overexpression of GNB2L1 and PKC-alpha suppressed CLOCK-BMAL1 transcriptional activity, and GNB2L1 stimulated phosphorylation of BMAL1 by PKC-alpha in vitro.
References
1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Patterson, R. L., van Rossum, D. B., Barrow, R. K., Snyder, S. H. RACK1 binds to inositol 1,4,5-triphosphate receptors and mediates Ca(2+) release. Proc. Nat. Acad. Sci. 101: 2328-2332, 2004. 3. Robles, M. S., Boyault, C., Knutti, D., Padmanabhan, K., Weitz, C. J. Identification of RACK1 and protein kinase C-alpha as integral components of the mammalian circadian clock. Science 327: 463-466, 2010.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,077 Da
NCBI Official Full Name
receptor of activated protein C kinase 1
NCBI Official Synonym Full Names
receptor for activated C kinase 1
NCBI Official Symbol
RACK1
NCBI Official Synonym Symbols
H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1
NCBI Protein Information
receptor of activated protein C kinase 1
UniProt Protein Name
Receptor of activated protein C kinase 1
UniProt Gene Name
RACK1
UniProt Synonym Gene Names
HLC-7
UniProt Entry Name
RACK1_HUMAN
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Product Notes
The RACK1 rack1 (Catalog #AAA46363) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-RACK1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RACK1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the RACK1 rack1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RACK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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