WB (Western Blot)
(Anti-Rad6/UBE2A rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa)
Rabbit anti-Human Rad6/UBE2A Polyclonal Antibody | anti-Rad6/UBE2A antibody
Anti-Rad6/UBE2A Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
Rad6/UBE2A, Antibody; Anti-Rad6/UBE2A Antibody, Rabbit Polyclonal; ubiquitin-conjugating enzyme E2A; Anti-HHR6A Antibody; Anti-MRXS30 Antibody; Anti-MRXSN Antibody; Anti-RAD6A Antibody; Anti-UBC2 Antibody; anti-Rad6/UBE2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Rad6/UBE2A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-Rad6/UBE2A antibody
IP (Immunoprecipitation), WB (Western Blot), ELISA
Immunogen
Recombinant Human Rad6/UBE2A Protein
Preparation
Produced in rabbits immunized with purified, recombinant Human Rad6/UBE2A (rh Rad6/UBE2A). Rad6/UBE2A specific IgG was purified by Human Rad6/UBE2A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-Rad6/UBE2A rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa)
WB (Western Blot)
(Anti-Rad6/UBE2A rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa)
IP (Immunoprecipitation)
(UBE2A was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate4 uL anti-UBE2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-UBE2A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 16 kDaObserved band size :16 kDa)
IP (Immunoprecipitation)
(UBE2A was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate4 uL anti-UBE2A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-UBE2A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 16 kDaObserved band size :16 kDa)
Related Product Information for anti-Rad6/UBE2A antibody
Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A), encoded by human DNA repair genes HHR6A, belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6/UBC2 gene, a member of the family of ubiquitin-conjugating enzymes. In vivo, HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition, with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity, while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is considered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family.
References
Nascimento RM, et al. (2006) UBE2A, which encodes a ubiquitin-conjugating enzyme, is mutated in a novel X-linked mental retardation syndrome. Am J Hum Genet. 79 (3): 549-55.
Koken MH, et al. (1992) Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31. Genomics. 12 (3): 447-53.
Sarcevic B, et al. (2002) Regulation of the ubiquitin-conjugating enzyme hHR6A by CDK-mediated phosphorylation. EMBO J. 21 (8): 2009-18.
Koken MH, et al. (1992) Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31. Genomics. 12 (3): 447-53.
Sarcevic B, et al. (2002) Regulation of the ubiquitin-conjugating enzyme hHR6A by CDK-mediated phosphorylation. EMBO J. 21 (8): 2009-18.
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Product Notes
The Rad6/UBE2A (Catalog #AAA258908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rad6/UBE2A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rad6/UBE2A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the Rad6/UBE2A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad6/UBE2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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