Rabbit RAG2 Polyclonal Antibody | anti-RAG2 antibody

RAG2 Polyclonal Antibody

Cat. Num. AAA280188

• Gene Names: RAG2; RAG-2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purification
• Synonyms: RAG2, Antibody; RAG2 Polyclonal Antibody; RAG-2; anti-RAG2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 527

• Applicable Applications for anti-RAG2 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human RAG2
• Calculated Molecular Weight: 59kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IF (Immunofluorescence)

(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using RAG2 antibody. Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells using RAG2 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using RAG2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Related Product Information for anti-RAG2 antibody

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Product Categories/Family for anti-RAG2 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 2498830
• NCBI GeneID: 5897
• UniProt Accession #: P55895; Q8TBL4; A8K9E9
• Molecular Weight: 527
• NCBI Official Full Name: V(D)J recombination-activating protein 2
• NCBI Official Synonym Full Names: recombination activating gene 2
• NCBI Official Symbol: RAG2
• NCBI Official Synonym Symbols: RAG-2
• NCBI Protein Information: V(D)J recombination-activating protein 2; V(D)J recombination-activating protein 2
• UniProt Protein Name: V(D)J recombination-activating protein 2
• UniProt Gene Name: RAG2
• UniProt Synonym Gene Names: RAG-2
• UniProt Entry Name: RAG2_HUMAN