Rabbit RANTES Polyclonal Antibody | anti-CCL5 antibody

RABBIT ANTI HUMAN RANTES

Cat. Num. AAA49937

• Gene Names: CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
• Applications: Western Blot, Immunohistochemistry, Functional Assay, ELISA
• Synonyms: RANTES, Antibody; RABBIT ANTI HUMAN RANTES; anti-CCL5 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0 mg/ml after reconstitution (varies by lot)
• Sequence Length: 91

• Applicable Applications for anti-CCL5 antibody: WB (Western Blot), IHC (Immunohistochemistry), FA (Functional Assay), ELISA
• Reconstitution: Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Application Data

(Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human RANTES (AAA49937) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Application Data

(Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human RANTES (AAA49937) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-CCL5 antibody

Rabbit anti Human RANTES antibody recognizes human RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells. RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.

NCBI and Uniprot Product Information

• NCBI GI #: 22538814
• NCBI GeneID: 6352
• NCBI Accession #: NP_002976.2
• NCBI GenBank Nucleotide #: NM_002985.2
• UniProt Accession #: P13501; O43646; Q0QVW8; Q4ZGJ1; Q9NYA2; Q9UBG2; Q9UC99
• Molecular Weight: 9,990 Da
• NCBI Official Full Name: C-C motif chemokine 5 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: CCL5
• NCBI Official Synonym Symbols: SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
• NCBI Protein Information: C-C motif chemokine 5; T-cell specific protein p288; beta-chemokine RANTES; eosinophil chemotactic cytokine; regulated upon activation, normally T-expressed, and presumably secreted; small inducible cytokine subfamily A (Cys-Cys), member 5
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: CCL5
• UniProt Synonym Gene Names: D17S136E; SCYA5; TCP228
• UniProt Entry Name: CCL5_HUMAN