WB (Western Blot)
(Detection of human RAP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RAP1 antibody AAA212210 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RAP1 was also immunoprecipitated by rabbit anti-RAP1 antibodies which recognizes a downstream epitope, and which is generated against full-length recombinant human RAP1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human RAP1 Polyclonal Antibody | anti-RAP1 antibody
Rabbit anti-RAP1 Antibody, Affinity Purified
Gene Names
TERF2IP; RAP1; DRIP5
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RAP1, Antibody; Rabbit anti-RAP1 Antibody, Affinity Purified; hRap1; TRF2-interacting telomeric RAP1 protein; TRF2-interacting telomeric protein 1; TERF2-interacting telomeric protein 1; telomeric repeat-binding factor 2-interacting protein 1; repressor/activator protein 1 homolog; RAP1; DRIP5; dopamine receptor-interacting protein 5; RAP1 homolog; Telomeric repeat-binding factor 2-interacting protein 1; TERF2 interacting protein; anti-RAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RAP1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 100 and 150
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RAP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RAP1 antibody AAA212210 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RAP1 was also immunoprecipitated by rabbit anti-RAP1 antibodies which recognizes a downstream epitope, and which is generated against full-length recombinant human RAP1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human RAP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RAP1 antibody AAA212210 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RAP1 was also immunoprecipitated by rabbit anti-RAP1 antibodies which recognizes a downstream epitope, and which is generated against full-length recombinant human RAP1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human RAP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAP1 (Cat. No. AAA212210 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human RAP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAP1 (Cat. No. AAA212210 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-RAP1 antibody
Repressor/activator protein 1 homolog (RAP1) acts both as a regulator of telomere function and as a transcriptional regulator. RAP1 is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, RAP1 is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NYB0].
Product Categories/Family for anti-RAP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,260 Da
NCBI Official Full Name
telomeric repeat-binding factor 2-interacting protein 1
NCBI Official Synonym Full Names
telomeric repeat binding factor 2, interacting protein
NCBI Official Symbol
TERF2IP
NCBI Official Synonym Symbols
RAP1; DRIP5
NCBI Protein Information
telomeric repeat-binding factor 2-interacting protein 1; hRap1; RAP1 homolog; TRF2-interacting telomeric protein 1; TERF2-interacting telomeric protein 1; repressor/activator protein 1 homolog; TRF2-interacting telomeric RAP1 protein; dopamine receptor in
UniProt Protein Name
Telomeric repeat-binding factor 2-interacting protein 1
UniProt Gene Name
TERF2IP
UniProt Synonym Gene Names
DRIP5; RAP1; TERF2-interacting telomeric protein 1; TRF2-interacting telomeric protein 1; RAP1 homolog; hRap1
UniProt Entry Name
TE2IP_HUMAN
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Product Notes
The RAP1 terf2ip (Catalog #AAA212210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RAP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RAP1 terf2ip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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