IP (Immunoprecipitation)
(Detection of human RAP74 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAP74 antibody AAA212000 used for IP at 3 ug/mg lysate. RAP74 was also immunoprecipitated by rabbit anti-RAP74 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RAP74, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human RAP74 Polyclonal Antibody | anti-RAP74 antibody
Rabbit anti-RAP74 Antibody, Affinity Purified
Gene Names
GTF2F1; BTF4; RAP74; TF2F1; TFIIF
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
RAP74, Antibody; Rabbit anti-RAP74 Antibody, Affinity Purified; RAP74; transcription initiation factor RAP74; transcription initiation factor IIF subunit alpha; TFIIF-alpha; TF2F1; general transcription factor IIF, polypeptide 1, 74kDa; general transcription factor IIF subunit 1; general transcription factor IIF 74 kDa subunit; BTF4; TFIIF; General transcription factor IIF subunit 1; anti-RAP74 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RAP74 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 275 and 325
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human RAP74 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAP74 antibody AAA212000 used for IP at 3 ug/mg lysate. RAP74 was also immunoprecipitated by rabbit anti-RAP74 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RAP74, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RAP74 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAP74 antibody AAA212000 used for IP at 3 ug/mg lysate. RAP74 was also immunoprecipitated by rabbit anti-RAP74 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RAP74, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human RAP74 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RAP74 (Cat. No. AAA212000 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RAP74 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RAP74 (Cat. No. AAA212000 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-RAP74 antibody
The general transcription factor, TFIIF, is composed of a heterodimer of a 74 kDa alpha subunit and a 30 kDa beta subunit. The alpha subunit of TFIIF is also called RAP74. In collaboration with TFIIB, TFIIF helps recruit RNA polymerase II to the pre-initiation complex (PIC).
Product Categories/Family for anti-RAP74 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,240 Da
NCBI Official Full Name
general transcription factor IIF subunit 1
NCBI Official Synonym Full Names
general transcription factor IIF, polypeptide 1, 74kDa
NCBI Official Symbol
GTF2F1
NCBI Official Synonym Symbols
BTF4; RAP74; TF2F1; TFIIF
NCBI Protein Information
general transcription factor IIF subunit 1; TFIIF-alpha; general transcription factor IIF 74 kDa subunit; transcription initiation factor IIF subunit alpha; transcription initiation factor RAP74
UniProt Protein Name
General transcription factor IIF subunit 1
UniProt Gene Name
GTF2F1
UniProt Synonym Gene Names
RAP74; TFIIF-alpha
UniProt Entry Name
T2FA_HUMAN
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Product Notes
The RAP74 gtf2f1 (Catalog #AAA212000) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RAP74 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAP74 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the RAP74 gtf2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP74, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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