WB (Western Blot)
(Detection of human RASAL1 by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, GaMG, and OVCAR-4 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RASAL1 antibody (AAA213470 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human RASAL1 Polyclonal Antibody | anti-RASAL1 antibody
Rabbit anti-RASAL1 Antibody, Affinity Purified
Gene Names
RASAL1; RASAL
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RASAL1, Antibody; Rabbit anti-RASAL1 Antibody, Affinity Purified; RASAL; rasGAP-activating-like protein 1; ras GTPase-activating-like protein; GAP1 like protein; RAS protein activator like 1; RasGAP-activating-like protein 1; anti-RASAL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RASAL1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 754 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RASAL1 by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, GaMG, and OVCAR-4 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RASAL1 antibody (AAA213470 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human RASAL1 by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, GaMG, and OVCAR-4 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RASAL1 antibody (AAA213470 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RASAL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RPMI-8226 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RASAL1 antibody (AAA213470 lot 1) used for IP at 1 ug per reaction. RASAL1 was also immunoprecipitated by rabbit anti-RASAL1 antibody BL24199. For blotting immunoprecipitated RASAL1, AAA213470 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RASAL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RPMI-8226 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RASAL1 antibody (AAA213470 lot 1) used for IP at 1 ug per reaction. RASAL1 was also immunoprecipitated by rabbit anti-RASAL1 antibody BL24199. For blotting immunoprecipitated RASAL1, AAA213470 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-RASAL1 antibody
RASAL1 is a member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, RASAL1 enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, RASAL1 is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described [provided by RefSeq, Jul 2010] [taken from NCBI Entrez Gene (Gene ID: 8437)].
Product Categories/Family for anti-RASAL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,016 Da
NCBI Official Full Name
rasGAP-activating-like protein 1 isoform 2
NCBI Official Synonym Full Names
RAS protein activator like 1 (GAP1 like)
NCBI Official Symbol
RASAL1
NCBI Official Synonym Symbols
RASAL
NCBI Protein Information
rasGAP-activating-like protein 1; GAP1 like protein
UniProt Protein Name
RasGAP-activating-like protein 1
UniProt Gene Name
RASAL1
UniProt Synonym Gene Names
RASAL
UniProt Entry Name
RASL1_HUMAN
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Product Notes
The RASAL1 rasal1 (Catalog #AAA213470) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RASAL1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RASAL1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RASAL1 rasal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RASAL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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