FCM/FACS (Flow Cytometry)
(RASGRP1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit RASGRP1 Polyclonal Antibody | anti-RASGRP1 antibody
RASGRP1 Antibody (Center)
Gene Names
RASGRP1; V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RASGRP1, Antibody; RASGRP1 Antibody (Center); RAS guanyl-releasing protein 1; Calcium and DAG-regulated guanine nucleotide exchange factor II; CalDAG-GEFII; Ras guanyl-releasing protein; RASGRP1; RASGRP; anti-RASGRP1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RASGRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-521 amino acids from the Central region of human RASGRP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
495-521
Applicable Applications for anti-RASGRP1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Functions as a calcium- and diacylglycerol(DAG)- regulated nucleotide exchange factorspecifically activating Ras through theexchange of bound GDP for GTP (PubMed: ,PubMed: ). Activatesthe Erk/MAP kinase cascade (PubMed: ). RegulatesT-cell/B-cell development, homeostasis anddifferentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras(PubMed: , PubMed: 12839994).Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (PubMed: ). Functions in mast cell degranulation and cytokinesecretion, regulating FcERI-evoked allergic responses (By similarity). May also function in differentiation of other cell types (PubMed: 12845332).
Cellular Location
Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Golgiapparatus membrane; Peripheral membraneprotein. Endoplasmic reticulum membrane;Peripheral membrane protein. Note=Foundboth in the cytosol and associated with membranes. Relocalization to the cellmembrane upon activation is F-actin-dependent. Translocates to the Golgiin response to phorbol ester or nerve growth factor. Localizes to somata and dendrites but not to axons of hippocampal pyramidal cells (By similarity).
Tissue Location
Expressed in brain with higher expression incerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level)Expressed in NK cells (at protein level)(PubMed:19933860)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(RASGRP1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(RASGRP1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(RASGRP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RASGRP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(RASGRP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RASGRP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(RASGRP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RASGRP1 antibody detected the RASGRP1 protein (arrow).)
WB (Western Blot)
(RASGRP1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RASGRP1 antibody detected the RASGRP1 protein (arrow).)
Product Categories/Family for anti-RASGRP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90402
NCBI Official Full Name
RAS guanyl-releasing protein 1 isoform b
NCBI Official Synonym Full Names
RAS guanyl releasing protein 1 (calcium and DAG-regulated)
NCBI Official Symbol
RASGRP1
NCBI Official Synonym Symbols
V; RASGRP; hRasGRP1; CALDAG-GEFI; CALDAG-GEFII
NCBI Protein Information
RAS guanyl-releasing protein 1
UniProt Protein Name
RAS guanyl-releasing protein 1
UniProt Gene Name
RASGRP1
UniProt Synonym Gene Names
RASGRP; CalDAG-GEFII
UniProt Entry Name
GRP1_HUMAN
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Product Notes
The RASGRP1 rasgrp1 (Catalog #AAA286358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 495-521. The RASGRP1 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RASGRP1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RASGRP1 rasgrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RASGRP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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