Rabbit RASGRP2 Polyclonal Antibody | anti-RASGRP2 antibody

RASGRP2 Antibody (N-term)

Cat. Num. AAA286653

• Gene Names: RASGRP2; CDC25L; CALDAG-GEFI
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Applications: Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
• Synonyms: RASGRP2, Antibody; RASGRP2 Antibody (N-term); RAS guanyl-releasing protein 2; Calcium and DAG-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25-like protein; hCDC25L; F25B33 kinase-like protein; RASGRP2; CDC25L; MCG7; anti-RASGRP2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human RASGRP2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 7-34
• Sequence Length: 610

• Applicable Applications for anti-RASGRP2 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
• Cellular Location: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome Cell projection, ruffle membrane; Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons (By similarity).
• Tissue Location: Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney
• Antigen Region: 7-34
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of RASGRP2 Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemisry)

(RASGRP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Anti-RASGRP2 Antibody (N-term) at 1:1000 dilution + human spleen lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L))

WB (Western Blot)

(RASGRP2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).)

Related Product Information for anti-RASGRP2 antibody

The protein encoded by this gene is a brain-enriched
nucleotide exchanged factor that contains an N-terminal GEF domain,
2 tandem repeats of EF-hand calcium-binding motifs, and a
C-terminal diacylglycerol/phorbol ester-binding domain. This
protein can activate small GTPases, including RAS and RAP1/RAS3.
The nucleotide exchange activity of this protein can be stimulated
by calcium and diacylglycerol. Three alternatively spliced
transcript variants encoding the same protein have been found for
this gene.

Product Categories/Family for anti-RASGRP2 antibody

Signal Transduction

References

Nagamine, K., et al. Biol. Pharm. Bull. 33(7):1138-1142(2010)
Katagiri, K., et al. J. Biol. Chem. 279(12):11875-11881(2004)
Eto, K., et al. Proc. Natl. Acad. Sci. U.S.A. 99(20):12819-12824(2002)
Toki, S., et al. J. Comp. Neurol. 437(4):398-407(2001)
Guo, F.F., et al. J. Biol. Chem. 276(27):25568-25581(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 149158727
• NCBI GeneID: 10235
• NCBI Accession #: NP_001092140.1; NP_001092141.1; NP_722541.1
• NCBI GenBank Nucleotide #: NM_001098670.1
• UniProt Accession #: Q7LDG7; O00538; Q9UL65; A6NDC7
• Molecular Weight: 69248
• NCBI Official Full Name: RAS guanyl-releasing protein 2
• NCBI Official Synonym Full Names: RAS guanyl releasing protein 2 (calcium and DAG-regulated)
• NCBI Official Symbol: RASGRP2
• NCBI Official Synonym Symbols: CDC25L; CALDAG-GEFI
• NCBI Protein Information: RAS guanyl-releasing protein 2
• UniProt Protein Name: RAS guanyl-releasing protein 2
• UniProt Gene Name: RASGRP2
• UniProt Synonym Gene Names: CDC25L; MCG7; CalDAG-GEFI; hCDC25L
• UniProt Entry Name: GRP2_HUMAN