WB (Western Blot)
(Detection of human and mouse RBBP4/RbAp48 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RBBP4/RbAp48 antibody AAA211355 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. RBBP4/RbAp48 was also immunoprecipitated by rabbit anti-RBBP4/RbAp48 antibody BL2987, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human, Mouse RbBP4 Polyclonal Antibody | anti-RbBP4 antibody
Rabbit anti-RbBP4 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RbBP4, Antibody; Rabbit anti-RbBP4 Antibody, Affinity Purified; CAF-1 subunit C; MSI1 protein homolog; retinoblastoma-binding protein p48; retinoblastoma-binding protein 4; RBBP-4; RBAP48; NURF55; nucleosome-remodeling factor subunit RBAP48; lin-53; histone-binding protein RBBP4; chromatin assembly factor/CAF-1 p48 subunit; chromatin assembly factor I p48 subunit; chromatin assembly factor 1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; Histone-binding protein RBBP4; RB binding protein 4, chromatin remodeling factor; anti-RbBP4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-RbBP4 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Orangutan
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RBBP4/RbAp48 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RBBP4/RbAp48 antibody AAA211355 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. RBBP4/RbAp48 was also immunoprecipitated by rabbit anti-RBBP4/RbAp48 antibody BL2987, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human and mouse RBBP4/RbAp48 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RBBP4/RbAp48 antibody AAA211355 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. RBBP4/RbAp48 was also immunoprecipitated by rabbit anti-RBBP4/RbAp48 antibody BL2987, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human RBBP4/RbAp48 by immunohistochemistry. Sample: FFPE section of human Ewing sarcoma. Antibody: Affinity purified rabbit anti-RBBP4/RbAp48 (Cat. No. AAA211355 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human RBBP4/RbAp48 by immunohistochemistry. Sample: FFPE section of human Ewing sarcoma. Antibody: Affinity purified rabbit anti-RBBP4/RbAp48 (Cat. No. AAA211355 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-RbBP4 antibody
RbBP4 is a member of the WD-repeat subfamily of proteins and is a component of multiple complexes involved in chromatin assembly, histone deacetylation, and transcriptional repression. RbBP4 has also been identified as a retinoblastoma (Rb) binding protein and plays a role in the repression of E2F-regulated genes in association with Rb and histone deacetylases.
Product Categories/Family for anti-RbBP4 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The RbBP4 rbbp4 (Catalog #AAA211355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RbBP4 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RbBP4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RbBP4 rbbp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RbBP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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