Rabbit anti-Mouse RBP3 Polyclonal Antibody | anti-RBP3 antibody

RBP3 Antibody (Center)

Cat. Num. AAA290047

• Gene Names: RBP3; IRBP; RBPI; RP66; D10S64; D10S65; D10S66
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RBP3, Antibody; RBP3 Antibody (Center); Retinol-binding protein 3; Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein; RBP3; anti-RBP3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RBP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-811 amino acids from the Central region of human RBP3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 784-811
• Sequence Length: 1,247

• Applicable Applications for anti-RBP3 antibody: ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Function: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.
• Cellular Location: Secreted, extracellular space, extracellular matrix, interphotoreceptor matrix. Note=Interphotoreceptor matrix that permeates the space between the retina and the contiguous layer of pigment epithelium cells contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-RBP3 Antibody (Center) at 1:2000 dilutionLane 1: mouse eyeball lysate Lane2: mouse lung lysate Lysates/proteins at 20 ?g per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 135 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RBP3 antibody

Interphotoreceptor retinol-binding protein is a large
glycoprotein known to bind retinoids and found primarily in the
interphotoreceptor matrix of the retina between the retinal pigment
epithelium and the photoreceptor cells. It is thought to transport
retinoids between the retinal pigment epithelium and the
photoreceptors, a critical role in the visual process.The human
IRBP gene is approximately 9.5 kbp in length and consists of four
exons separated by three introns. The introns are 1.6-1.9 kbp long.
The gene is transcribed by photoreceptor and retinoblastoma cells
into an approximately 4.3-kilobase mRNA that is translated and
processed into a glycosylated protein of 135,000 Da. The amino acid
sequence of human IRBP can be divided into four contiguous homology
domains with 33-38% identity, suggesting a series of gene
duplication events. In the gene, the boundaries of these domains
are not defined by exon-intron junctions, as might have been
expected. The first three homology domains and part of the fourth
are all encoded by the first large exon, which is 3,180 base pairs
long. The remainder of the fourth domain is encoded in the last
three exons, which are 191, 143, and approximately 740 base pairs
long, respectively.

Product Categories/Family for anti-RBP3 antibody

Cardiovascular; Neuroscience; Signal Transduction

References

Garcia-Ramirez, M., et al. Diabetologia 52(12):2633-2641(2009)
den Hollander, A.I., et al. Invest. Ophthalmol. Vis. Sci. 50(4):1864-1872(2009)
Jin, M., et al. J. Neurosci. 29(5):1486-1495(2009)
Descamps, F.J., et al. J. Cell. Mol. Med. 12 (6A), 2449-2456 (2008) :
Howard, O.M., et al. Blood 105(11):4207-4214(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 4506453
• NCBI GeneID: 5949
• NCBI Accession #: NP_002891.1
• NCBI GenBank Nucleotide #: NM_002900.2
• UniProt Accession #: P10745; Q0QD34; Q5VSR0; Q8IXN0
• Molecular Weight: 135363
• NCBI Official Full Name: retinol-binding protein 3
• NCBI Official Synonym Full Names: retinol binding protein 3, interstitial
• NCBI Official Symbol: RBP3
• NCBI Official Synonym Symbols: IRBP; RBPI; RP66; D10S64; D10S65; D10S66
• NCBI Protein Information: retinol-binding protein 3
• UniProt Protein Name: Retinol-binding protein 3
• UniProt Gene Name: RBP3
• UniProt Synonym Gene Names: IRBP
• UniProt Entry Name: RET3_HUMAN