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WB (Western Blot)
(Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using RBX1 Antibody (C-term). AAA285048 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit RBX1 Polyclonal Antibody | anti-RBX1 antibody
RBX1 Antibody (C-term)
Gene Names
RBX1; ROC1; RNF75; BA554C12.1
Reactivity
Human (Predicted Reactivity: C Elegans, Mouse)
Applications
Immunohistochemistry, Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RBX1, Antibody; RBX1 Antibody (C-term); E3 ubiquitin-protein ligase RBX1; 632-; Protein ZYP; RING finger protein 75; RING-box protein 1; Rbx1; Regulator of cullins 1; N-terminally processed; RBX1; RNF75; ROC1; anti-RBX1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: C Elegans, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RBX1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 74-108 amino acids from the C-terminal region of human RBX1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
108
Applicable Applications for anti-RBX1 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using RBX1 Antibody (C-term). AAA285048 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using RBX1 Antibody (C-term). AAA285048 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded H. colon section using RBX1 Antibody (C-term). AAA285048 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded H. colon section using RBX1 Antibody (C-term). AAA285048 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded M. testis section using RBX1 Antibody (C-term). AAA285048 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded M. testis section using RBX1 Antibody (C-term). AAA285048 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IF (Immunofluorescence)
(Fluorescent image of HeLa cells stained with RBX1 Antibody (C-term). AAA285048 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of HeLa cells stained with RBX1 Antibody (C-term). AAA285048 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-RBX1 antibody
E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.
Product Categories/Family for anti-RBX1 antibody
References
Ohta T.,et al.Mol. Cell 3:535-541(1999).
Kamura T.,et al.Science 284:657-661(1999).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham I.,et al.Nature 402:489-495(1999).
Kamura T.,et al.Science 284:657-661(1999).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham I.,et al.Nature 402:489-495(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12274
NCBI Official Full Name
E3 ubiquitin-protein ligase RBX1
NCBI Official Synonym Full Names
ring-box 1, E3 ubiquitin protein ligase
NCBI Official Symbol
RBX1
NCBI Official Synonym Symbols
ROC1; RNF75; BA554C12.1
NCBI Protein Information
E3 ubiquitin-protein ligase RBX1
UniProt Protein Name
E3 ubiquitin-protein ligase RBX1
UniProt Gene Name
RBX1
UniProt Synonym Gene Names
RNF75; ROC1; Rbx1
UniProt Entry Name
RBX1_HUMAN
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Product Notes
The RBX1 rbx1 (Catalog #AAA285048) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RBX1 Antibody (C-term) reacts with Human (Predicted Reactivity: C Elegans, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's RBX1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RBX1 rbx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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