IF (Immunofluorescence)
(Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly.)
Rabbit RCAN1 Polyclonal Antibody | anti-RCAN1 antibody
RCAN1 Antibody (N-term)
Gene Names
RCAN1; CSP1; DSC1; RCN1; DSCR1; MCIP1; ADAPT78
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RCAN1, Antibody; RCAN1 Antibody (N-term); Calcipressin-1; Adapt78; Down syndrome critical region protein 1; Myocyte-enriched calcineurin-interacting protein 1; MCIP1; Regulator of calcineurin 1; RCAN1; ADAPT78; CSP1; DSC1; DSCR1; anti-RCAN1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RCAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-68 amino acids from the N-terminal region of human RCAN1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (varies by lot)
Sequence Positions
41-68
Sequence Length
117
Applicable Applications for anti-RCAN1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly.)
WB (Western Blot)
(Western blot analysis of RCAN1 (arrow) using rabbit polyclonal RCAN1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.)
WB (Western Blot)
(Western blot analysis of RCAN1 (arrow) using rabbit polyclonal RCAN1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.)
WB (Western Blot)
(RCAN1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RCAN1 antibody detected the RCAN1 protein (arrow).)
WB (Western Blot)
(RCAN1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RCAN1 antibody detected the RCAN1 protein (arrow).)
Related Product Information for anti-RCAN1 antibody
The protein encoded by this gene interacts with
calcineurin A and inhibits calcineurin-dependent signaling
pathways, possibly affecting central nervous system development.
This gene is located in the minimal candidate region for the Down
syndrome phenotype, and is overexpressed in the brain of Down
syndrome fetuses. Chronic overexpression of this gene may lead to
neurofibrillary tangles such as those associated with Alzheimer
disease. Three transcript variants encoding three different
isoforms have been found for this gene.
calcineurin A and inhibits calcineurin-dependent signaling
pathways, possibly affecting central nervous system development.
This gene is located in the minimal candidate region for the Down
syndrome phenotype, and is overexpressed in the brain of Down
syndrome fetuses. Chronic overexpression of this gene may lead to
neurofibrillary tangles such as those associated with Alzheimer
disease. Three transcript variants encoding three different
isoforms have been found for this gene.
Product Categories/Family for anti-RCAN1 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Tam, G.W., et al. Biochem. Soc. Trans. 38(2):445-451(2010)
Lee, M.Y., et al. Hum. Mol. Genet. 19(3):468-479(2010)
Sales, K.J., et al. Am. J. Pathol. 176(1):435-445(2010)
Sales, K.J., et al. Biochim. Biophys. Acta 1793(12):1917-1928(2009)
Tam, G.W., et al. Biochem. Soc. Trans. 38(2):445-451(2010)
Lee, M.Y., et al. Hum. Mol. Genet. 19(3):468-479(2010)
Sales, K.J., et al. Am. J. Pathol. 176(1):435-445(2010)
Sales, K.J., et al. Biochim. Biophys. Acta 1793(12):1917-1928(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28079
NCBI Official Full Name
calcipressin-1 isoform a
NCBI Official Synonym Full Names
regulator of calcineurin 1
NCBI Official Symbol
RCAN1
NCBI Official Synonym Symbols
CSP1; DSC1; RCN1; DSCR1; MCIP1; ADAPT78
NCBI Protein Information
calcipressin-1
UniProt Protein Name
Calcipressin-1
UniProt Gene Name
RCAN1
UniProt Synonym Gene Names
ADAPT78; CSP1; DSC1; DSCR1; MCIP1
UniProt Entry Name
RCAN1_HUMAN
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Product Notes
The RCAN1 rcan1 (Catalog #AAA290327) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 41-68. The RCAN1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's RCAN1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RCAN1 rcan1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RCAN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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