WB (Western Blot)
(Detection of human and mouse RCOR3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCOR3 antibody AAA211387 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A) and 10 seconds (B).)
Rabbit anti-Human, Mouse RCOR3 Polyclonal Antibody | anti-RCOR3 antibody
Rabbit anti-RCOR3 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RCOR3, Antibody; Rabbit anti-RCOR3 Antibody, Affinity Purified; REST corepressor 3; anti-RCOR3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-RCOR3 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 445 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RCOR3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCOR3 antibody AAA211387 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human and mouse RCOR3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCOR3 antibody AAA211387 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A) and 10 seconds (B).)
IHC (Immunohiostchemistry)
(Detection of human RCOR3 by immunohistochemistry. Sample: FFPE sections of human testicular seminoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti-RCOR3 (Cat. No. AAA211387 Lot1) used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100)
IHC (Immunohiostchemistry)
(Detection of human RCOR3 by immunohistochemistry. Sample: FFPE sections of human testicular seminoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti-RCOR3 (Cat. No. AAA211387 Lot1) used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100)
ICC (Immunocytochemistry)
(Detection of human RCOR3 by immunocytochemistry. Sample: FFPE section of NBF-fixed HeLa Cells Antibody: Affinity purified rabbit anti-RCOR3 (Cat. No. AAA211387 Lot1) used at a dilution of 1:80 (2.5 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 488 Conjugated used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human RCOR3 by immunocytochemistry. Sample: FFPE section of NBF-fixed HeLa Cells Antibody: Affinity purified rabbit anti-RCOR3 (Cat. No. AAA211387 Lot1) used at a dilution of 1:80 (2.5 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 488 Conjugated used at a dilution of 1:100.)
Related Product Information for anti-RCOR3 antibody
RCOR3 is a member of the coREST family that functions as a co-repressor for REST (RE1-silencing transcription factor) to regulate neuronal gene expression and neuronal stem cell fate. The coREST family includes RCOR1, also known as coREST, RCOR2, and RCOR3. REST complexes regulate gene expression by modifying chromatin to create a repressive epigenetic environment.
Product Categories/Family for anti-RCOR3 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The RCOR3 rcor3 (Catalog #AAA211387) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RCOR3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RCOR3 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RCOR3 rcor3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RCOR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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