WB (Western Blot)
(Detection of mouse REA by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, and TCMK-1 cells. Antibody: Affinity purified rabbit anti-REA antibody (AAA211077 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse REA Polyclonal Antibody | anti-REA antibody
Rabbit anti-REA Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
REA, Antibody; Rabbit anti-REA Antibody, Affinity Purified; hBAP; repressor of estrogen receptor activity; REA; prohibitin-2; BAP; p22; D-prohibitin; B-cell receptor-associated protein BAP37; B-cell associated protein; BCAP37; Bap37; PNAS-141; Prohibitin-2; prohibitin 2; anti-REA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-REA antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 249 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse REA by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, and TCMK-1 cells. Antibody: Affinity purified rabbit anti-REA antibody (AAA211077 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse REA by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, and TCMK-1 cells. Antibody: Affinity purified rabbit anti-REA antibody (AAA211077 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human REA by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-REA antibody (AAA211077 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human REA by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-REA antibody (AAA211077 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human REA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REA antibody (AAA211077 lot 3) used for IP at 20 ul per reaction. REA was also immunoprecipitated by a previous lot of this antibody (AAA211077 lot 2) and another rabbit anti-REA antibody . For blotting immunoprecipitated REA, AAA211077 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human REA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REA antibody (AAA211077 lot 3) used for IP at 20 ul per reaction. REA was also immunoprecipitated by a previous lot of this antibody (AAA211077 lot 2) and another rabbit anti-REA antibody . For blotting immunoprecipitated REA, AAA211077 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-REA antibody
REA (repressor of estrogen receptor activity), also known as prohibitin 2, has been shown to exhibit a variety of functions that includes mitochondrial chaperone activity, cell cycle regulation, and transcriptional regulation. REA localizes to the mitochondria and associates with mitochondrial complex I where it may play a role in mitochondrial metabolism. REA interacts with pRb to inhibit E2F-mediated transcription as well as the estrogen receptor to repress estrogen-dependent transcription via recruitment of histone deacetylases.
Product Categories/Family for anti-REA antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The REA phb2 (Catalog #AAA211077) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-REA Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's REA can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the REA phb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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