FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEL cells using anti-RED1/ADARB1 antibody (AAA126178).Overlay histogram showing HEL cells stained with AAA126178 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RED1/ADARB1 Antibody (AAA126178, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit RED1/ADARB1 Polyclonal Antibody | anti-ADARB1 antibody
Anti-RED1/ADARB1 Antibody Picoband
Gene Names
ADARB1; RED1; ADAR2; DRABA2; DRADA2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
RED1/ADARB1, Antibody; Anti-RED1/ADARB1 Antibody Picoband; anti-ADARB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Natural and recombinant Mouse Col18a1
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-ADARB1 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Assay Type
Sandwich
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEL cells using anti-RED1/ADARB1 antibody (AAA126178).Overlay histogram showing HEL cells stained with AAA126178 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RED1/ADARB1 Antibody (AAA126178, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEL cells using anti-RED1/ADARB1 antibody (AAA126178).Overlay histogram showing HEL cells stained with AAA126178 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RED1/ADARB1 Antibody (AAA126178, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-RED1/ADARB1 antibody (AAA126178).Overlay histogram showing 293T cells stained with AAA126178 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RED1/ADARB1 Antibody (AAA126178, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-RED1/ADARB1 antibody (AAA126178).Overlay histogram showing 293T cells stained with AAA126178 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RED1/ADARB1 Antibody (AAA126178, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of RED1/ADARB1 using anti-RED1/ADARB1 antibody (AAA126178).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SiHa whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human HEL whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RED1/ADARB1 antigen affinity purified polyclonal antibody (#AAA126178) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RED1/ADARB1 at approximately 90 kDa. The expected band size for RED1/ADARB1 is at 81 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of RED1/ADARB1 using anti-RED1/ADARB1 antibody (AAA126178).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SiHa whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human HEL whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RED1/ADARB1 antigen affinity purified polyclonal antibody (#AAA126178) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RED1/ADARB1 at approximately 90 kDa. The expected band size for RED1/ADARB1 is at 81 kDa.)
Related Product Information for anti-ADARB1 antibody
Background: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Principle of the Assay: The Quick Picokine™ Mouse Col18a1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Mouse Col18a1 with immunogen: Expression system for standard: NS0; Immunogen sequence: H1591-K1774. To measure Mouse Col18a1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Mouse Col18a1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Col18a1 in the sample.
Principle of the Assay: The Quick Picokine™ Mouse Col18a1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Mouse Col18a1 with immunogen: Expression system for standard: NS0; Immunogen sequence: H1591-K1774. To measure Mouse Col18a1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Mouse Col18a1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Col18a1 in the sample.
Product Categories/Family for anti-ADARB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80,763 Da
NCBI Official Full Name
double-stranded RNA-specific editase 1 isoform 1
NCBI Official Synonym Full Names
adenosine deaminase, RNA-specific, B1
NCBI Official Symbol
ADARB1
NCBI Official Synonym Symbols
RED1; ADAR2; DRABA2; DRADA2
NCBI Protein Information
double-stranded RNA-specific editase 1; RNA editase; RED1 homolog; RNA-editing enzyme 1; RNA editing deaminase 1; RNA-editing deaminase 1; dsRNA adenosine deaminase DRADA2; adenosine deaminase, RNA-specific, B1 (RED1 homolog rat); adenosine deaminase, RNA
UniProt Protein Name
Double-stranded RNA-specific editase 1
UniProt Gene Name
ADARB1
UniProt Synonym Gene Names
ADAR2; DRADA2; RED1
UniProt Entry Name
RED1_HUMAN
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Product Notes
The ADARB1 adarb1 (Catalog #AAA126178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RED1/ADARB1 Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RED1/ADARB1 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the ADARB1 adarb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RED1/ADARB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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