Goat anti-Human, Mouse RelA Polyclonal Antibody | anti-RelA antibody

Goat anti-RelA Antibody, Affinity Purified

Cat. Num. AAA212397

• Reactivity: Human, Mouse
• Applications: Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: RelA, Antibody; Goat anti-RelA Antibody, Affinity Purified; p65; NF-kappa-B transcription factor p65; v-rel reticuloendotheliosis viral oncogene homolog A; v-rel avian reticuloendotheliosis viral oncogene homolog A; transcription factor p65; NF-kappa-B p65delta3; NFKB3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; Transcription factor p65; RELA proto-oncogene, NF-kB subunit; anti-RelA antibody

• Host: Goat
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-RelA antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 501 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human RelA by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified goat anti-RelA antibody AAA212397 (lot AAA212397-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

WB (Western Blot)

(Detection of mouse RelA by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat anti-RelA antibody AAA212397 (lot AAA212397-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human RelA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-RelA antibody AAA212397 (lot AAA212397-1) used for IP at 6 ug/mg lysate. RelA was also immunoprecipitated by rabbit anti-RelA antibody For blotting immunoprecipitated RelA, AAA212397 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

Related Product Information for anti-RelA antibody

RelA is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. Each Rel member bears a characteristic 300 amino acid N-terminal Rel homology domain (RHD) that functions to mediate DNA binding, dimerization, nuclear localization, and inhibitor association.

Product Categories/Family for anti-RelA antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GeneID: 5970
• UniProt Accession #: Q04206
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN