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WB (Western Blot)
(Anti-NFkb p 65 Rel A Antibody - Western Blot. Western blot of HeLa cell extract. A predominant band ~65 kD (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.)
Rabbit RELA / NFKB p65 Polyclonal Antibody | anti-RELA antibody
Rabbit Polyclonal to Human RELA / NFKB p65
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunoprecipitation, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Delipidated and defibrinated
Synonyms
RELA / NFKB p65, Antibody; Rabbit Polyclonal to Human RELA / NFKB p65; Anti-RELA / NFKB p65 Antibody (C-Terminus) IHC-plus; RELA; NF-kappa-B p65delta3; NFKB3; p65; Transcription factor p65; Human RELA; NFKB p65; anti-RELA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Purity/Purification
Delipidated and defibrinated
Form/Format
Containing 0.01% sodium azide
Concentration
80 mg/ml (varies by lot)
Sequence Length
551
Applicable Applications for anti-RELA antibody
ELISA, IP (Immunoprecipitation), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Human
Antigen Modification
C-Terminus
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-NFkb p 65 Rel A Antibody - Western Blot. Western blot of HeLa cell extract. A predominant band ~65 kD (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.)
WB (Western Blot)
(Anti-NFkb p 65 Rel A Antibody - Western Blot. Western blot of HeLa cell extract. A predominant band ~65 kD (arrowhead) corresponding to NFkb p65 Rel A is observed. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.)
WB (Western Blot)
(Immunoblot of HeLa cell extract using anti-NFkb p65 (RelA) at a 1:5000 dilution. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed)
WB (Western Blot)
(Immunoblot of HeLa cell extract using anti-NFkb p65 (RelA) at a 1:5000 dilution. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed)
IHC (Immunohiostchemistry)
(Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
IHC (Immunohiostchemistry)
(Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
IHC (Immunohistochemistry)
(Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
IHC (Immunohistochemistry)
(Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
Related Product Information for anti-RELA antibody
NFKB1 or NFKB2 bind to REL, RELA, or RELB to form the NFKB complex. The NFKB1/RELA heterodimer is the most abundant form of NFKB. The NFKB complex is inactivated by I-kappa-B proteins that keep it trapped in the cytoplasm. Phosphorylation of serine residues on the I-kappa B proteins mark them for ubiquitination and destruction, and NFKB translocates to the nucleus where it binds DNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,910 Da
NCBI Official Full Name
transcription factor p65 isoform 1
NCBI Official Synonym Full Names
v-rel avian reticuloendotheliosis viral oncogene homolog A
NCBI Official Symbol
RELA
NCBI Official Synonym Symbols
p65; NFKB3
NCBI Protein Information
transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
UniProt Protein Name
Transcription factor p65
UniProt Gene Name
RELA
UniProt Synonym Gene Names
NFKB3
UniProt Entry Name
TF65_HUMAN
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Product Notes
The RELA rela (Catalog #AAA50695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human RELA / NFKB p65 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RELA / NFKB p65 can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RELA / NFKB p65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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