WB (Western Blot)
(Detection of mouse RENT2 by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (50ug), TCMK-1 (50ug), and 4T1 (50ug) cells. Antibodies: Affinity purified rabbit anti-RENT2 antibody AAA212382 (lot AAA212382-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse RENT2 Polyclonal Antibody | anti-RENT2 antibody
Rabbit anti-RENT2 Antibody, Affinity Purified
Gene Names
UPF2; HUPF2; RENT2; smg-3
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RENT2, Antibody; Rabbit anti-RENT2 Antibody, Affinity Purified; FRS2/UPF2/LEMD3 fusion; smg-3; yeast Upf2p homolog; up-frameshift suppressor 2 homolog; UPF2 regulator of nonsense transcripts homolog; smg-3 homolog, nonsense mediated mRNA decay factor; regulator of nonsense transcripts 2; nonsense mRNA reducing factor 2; LEMD3/UPF2 fusion; HUPF2; RENT2; Regulator of nonsense transcripts 2; UPF2, regulator of nonsense mediated mRNA decay; anti-RENT2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RENT2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse RENT2 by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (50ug), TCMK-1 (50ug), and 4T1 (50ug) cells. Antibodies: Affinity purified rabbit anti-RENT2 antibody AAA212382 (lot AAA212382-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse RENT2 by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (50ug), TCMK-1 (50ug), and 4T1 (50ug) cells. Antibodies: Affinity purified rabbit anti-RENT2 antibody AAA212382 (lot AAA212382-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human RENT2 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-RENT2 antibody AAA212382 (lot AAA212382-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human RENT2 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-RENT2 antibody AAA212382 (lot AAA212382-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RENT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-RENT2 antibody AAA212382 (lot AAA212382-1) used for IP at 6 ug/mg lysate. For blotting immunoprecipitated RENT2, AAA212382 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RENT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-RENT2 antibody AAA212382 (lot AAA212382-1) used for IP at 6 ug/mg lysate. For blotting immunoprecipitated RENT2, AAA212382 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-RENT2 antibody
Regulator of nonsense transcripts 2 (RENT2), also known as UPF2, is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). RENT2 is recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B, RENT2 stimulates both ATPase and RNA helicase activities of UPF1. RENT2 binds spliced mRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HAU5].
Product Categories/Family for anti-RENT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
147,810 Da
NCBI Official Full Name
regulator of nonsense transcripts 2
NCBI Official Synonym Full Names
UPF2 regulator of nonsense transcripts homolog (yeast)
NCBI Official Symbol
UPF2
NCBI Official Synonym Symbols
HUPF2; RENT2; smg-3
NCBI Protein Information
regulator of nonsense transcripts 2; yeast Upf2p homolog; nonsense mRNA reducing factor 2; up-frameshift suppressor 2 homolog; smg-3 homolog, nonsense mediated mRNA decay factor
UniProt Protein Name
Regulator of nonsense transcripts 2
UniProt Gene Name
UPF2
UniProt Synonym Gene Names
KIAA1408; RENT2; hUpf2
UniProt Entry Name
RENT2_HUMAN
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Product Notes
The RENT2 upf2 (Catalog #AAA212382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RENT2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RENT2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RENT2 upf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RENT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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