WB (Western Blot)
(Western blot analysis of extracts of Rat Heart tissue sample,using Phospho-REPS1 (Ser272) Antibody.)
Rabbit REPS1 Polyclonal Antibody | anti-REPS1 antibody
Phospho-REPS1 (Ser272) Antibody
Gene Names
REPS1; NBIA7; RALBP1
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
REPS1, Antibody; Phospho-REPS1 (Ser272) Antibody; RALBP1; RalBP1 associated Eps domain containing protein 1; RalBP1 interacting protein 1; RalBP1-associated Eps domain-containing protein 1; RalBP1-interacting protein 1; REPS1; REPS1_HUMAN; anti-REPS1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-REPS1 (Ser272) Antibody detects endogenous levels of REPS1 only when phosphorylated at Ser272.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
769
Applicable Applications for anti-REPS1 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human REPS1 around the phosphorylation site of Ser272.
Tissue Specificity
Widely expressed with highest levels in heart and testis.
Subcellular Location
Membrane > Clathrin-Coated Pit. Colocalize with ITSN1 at the plasma membrane in structures that are most probably Clathrin-Coated Pits.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of Rat Heart tissue sample,using Phospho-REPS1 (Ser272) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of Rat Heart tissue sample,using Phospho-REPS1 (Ser272) Antibody.)
IHC (Immunohiostchemistry)
(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-REPS1 antibody
Description: May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.
Post Translational Modifications: EGF stimulates phosphorylation on Tyr-residues.
Subunit Structure: Homodimer (Potential). Interacts with RAB11FIP2. Interacts with RALBP1, CRK and GRB2. Binding to RALBP1 does not affect its Ral-binding activity. Forms a complex with the SH3 domains of CRK and GRB2 which may link it to an EGF-responsive tyrosine kinase (By similarity). Interacts with AMPH, ITSN1 (via SH3 domains) and SGIP1; may be involved in clathrin-mediated endocytosis.
Post Translational Modifications: EGF stimulates phosphorylation on Tyr-residues.
Subunit Structure: Homodimer (Potential). Interacts with RAB11FIP2. Interacts with RALBP1, CRK and GRB2. Binding to RALBP1 does not affect its Ral-binding activity. Forms a complex with the SH3 domains of CRK and GRB2 which may link it to an EGF-responsive tyrosine kinase (By similarity). Interacts with AMPH, ITSN1 (via SH3 domains) and SGIP1; may be involved in clathrin-mediated endocytosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 80KD
Predicted: 87 kDa
Predicted: 87 kDa
NCBI Official Full Name
ralBP1-associated Eps domain-containing protein 1 isoform b
NCBI Official Synonym Full Names
RALBP1 associated Eps domain containing 1
NCBI Official Symbol
REPS1
NCBI Official Synonym Symbols
NBIA7; RALBP1
NCBI Protein Information
ralBP1-associated Eps domain-containing protein 1
UniProt Protein Name
RalBP1-associated Eps domain-containing protein 1
UniProt Gene Name
REPS1
UniProt Entry Name
REPS1_HUMAN
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Product Notes
The REPS1 reps1 (Catalog #AAA328546) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-REPS1 (Ser272) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's REPS1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the REPS1 reps1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REPS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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