IP (Immunoprecipitation)
(Detection of human REST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REST antibody AAA211008 (lot AAA211008-2) used for IP at 6 ug per reaction. For blotting immunoprecipitated REST, AAA211008 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human REST Polyclonal Antibody | anti-REST antibody
Rabbit anti-REST Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
REST, Antibody; Rabbit anti-REST Antibody, Affinity Purified; NRSF; XBR; X2 box repressor; WT6; DFNA27; RE1-silencing transcription factor; neuron restrictive silencer factor; neural-restrictive silencer factor; HGF5; GINGF5; repressor binding to the X2 box; RE1 silencing transcription factor; anti-REST antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-REST antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 800 and 850
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human REST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REST antibody AAA211008 (lot AAA211008-2) used for IP at 6 ug per reaction. For blotting immunoprecipitated REST, AAA211008 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human REST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REST antibody AAA211008 (lot AAA211008-2) used for IP at 6 ug per reaction. For blotting immunoprecipitated REST, AAA211008 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human REST by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-REST (Cat. No. AAA211008 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human REST by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-REST (Cat. No. AAA211008 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-REST antibody
RE1-Silencing Transcription Factor (REST) is a transcriptional repressor that silences neuronal gene expression in non-neuronal cells by binding to the neuron-restrictive silencer element (NRSE). REST is a Kruppel-type zinc finger transcription factor that mediates repression through its interaction with the mSin3 and CoREST corepressors and the HDAC1 and HDAC2 histone deacetylases.
Product Categories/Family for anti-REST antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The REST rest (Catalog #AAA211008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-REST Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's REST can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the REST rest for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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