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IHC (Immunohistochemistry)
(Immunogold labeling of epithelium cells, in vivo injected with RFP expressing vector;)
Goat RFP Polyclonal Antibody | anti-RFP antibody
RFP Polyclonal Antibody
Reactivity
Reacts with Transfected cells proteins.
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
This antibody is epitope-affinity purified from goat antiserum.
Synonyms
RFP, Antibody; RFP Polyclonal Antibody; Cherry fluorescent protein; dsRed; red fluorescent protein; tdTomato antibody; anti-RFP antibody
Host
Goat
Reactivity
Reacts with Transfected cells proteins.
Clonality
Polyclonal
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Purity/Purification
This antibody is epitope-affinity purified from goat antiserum.
Form/Format
Polyclonal antibody supplied as a 100 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Applicable Applications for anti-RFP antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry Formalin/Paraffin (IHC-F/IHC-P)
Application Notes
Western blot: 1:500-1:2,000
Immunofluorescence: 1:50-1:500
Immunohistochemistry (paraffin): 1:50-1:500
Immunohistochemistry (frozen): 1:50-1:500
Immunofluorescence: 1:50-1:500
Immunohistochemistry (paraffin): 1:50-1:500
Immunohistochemistry (frozen): 1:50-1:500
Immunogen
Purified recombinant RFP peptide produced in E Coli.
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
For continuous use, store at 2-8 degrees C for one-two days. For extended storage, store in -20 degrees C freezer. Working dilution samples should be discarded if not used within 12 hours.
IHC (Immunohistochemistry)
(Immunogold labeling of epithelium cells, in vivo injected with RFP expressing vector;)
IHC (Immunohistochemistry)
(Immunogold labeling of epithelium cells, in vivo injected with RFP expressing vector;)
WB (Western Blot)
(Anti-RFP Ab at 1/2,000 dilution; 293HEK cells transduced with RFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
WB (Western Blot)
(Anti-RFP Ab at 1/2,000 dilution; 293HEK cells transduced with RFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
IF (Immunofluorescence)
(Immunofluorescence – anti-RFP Ab in 293HEK cells transfected with RFP at 1/50 dilution; cells were fixed with 4% of PFA;)
IF (Immunofluorescence)
(Immunofluorescence – anti-RFP Ab in 293HEK cells transfected with RFP at 1/50 dilution; cells were fixed with 4% of PFA;)
IF (Immunofluorescence)
(Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing RFP in neurons (DyGlutRFP) using 1st Ab anti-RFP at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight®488 at 1/500;)
IF (Immunofluorescence)
(Immunofluorescence in Drosophila larvae NMJ muscle 6/7 expressing RFP in neurons (DyGlutRFP) using 1st Ab anti-RFP at 1/1,000 and 2nd Ab anti-goat IgY conjugated to DyLight®488 at 1/500;)
Reactivity Chart
(+++ excellent, ++ good, + poor, ND - not determined)
Reactivity Chart
(+++ excellent, ++ good, + poor, ND - not determined)
Related Product Information for anti-RFP antibody
Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.
Similar Products
Product Notes
The RFP (Catalog #AAA13877) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The RFP Polyclonal Antibody reacts with Reacts with Transfected cells proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's RFP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry Formalin/Paraffin (IHC-F/IHC-P). Western blot: 1:500-1:2,000 Immunofluorescence: 1:50-1:500 Immunohistochemistry (paraffin): 1:50-1:500 Immunohistochemistry (frozen): 1:50-1:500. Researchers should empirically determine the suitability of the RFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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