Rabbit RhoA (Ser-188) Polyclonal Antibody

RhoA (Ser-188), phospho-specific

Cat. Num. AAA71530

• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: RhoA (Ser-188), Antibody; RhoA (Ser-188), phospho-specific; Gb; p21; anti-RhoA (Ser-188) antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: This antibody was affinity purified using phospho-Rho (Ser-188) peptide (without carrier). The antibody detects a RhoA GST fusion protein when phosphorylated with PKA, but does not detect the unphosphorylated recombinant protein.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-RhoA (Ser-188) antibody: WB (Western Blot), ELISA
• Immunogen: Phospho-RhoA (Ser-188) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 188 of human RhoA. This peptide sequence is highly conserved in rat and mouse RhoA, but is not found in RhoB or RhoC proteins.
• Buffer: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffer saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. Store -20 degree C. Stable for 1 year.
• Preparation and Storage: Store at -20 degree C.

WB (Western Blot)

(Western blot analysis of human RhoA GST fusion recombinant unphosphorylated (lanes 1 & 3) or phosphorylated with PKA (lanes 2, 4, 5 & 6). The blots were probed with anti-Rho ( lanes 1 & 2) or with anti-RhoA (Ser-188) (AAA71530; lanes 3-6). The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-RhoA (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6).)

Related Product Information for anti-RhoA (Ser-188) antibody

Rho (A, B, & C) proteins are members of the Ras superfamily of GTPases. These proteins regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and an inactive GDPbound form. Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The Rho proteins RhoA, RhoB, and RhoC are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser-188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser-188 is an important site for negative regulation of RhoA activity.

References

• Lang, P. et al. (1996) EMBO J. 15:510.
• Dong, J.M. et al. (1998) J. Biol. Chem. 273(35):22554.
• Sawada, N. et al. (2001) Biochem. Biophys. Res. Commun. 280:798.
• Ellerbroek, S.M. et al. (2003) J. Biol. Chem. 278(21):19023.

NCBI and Uniprot Product Information

• UniProt Accession #: P06749