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WB (Western Blot)
(Detection of mouse Rictor by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody AAA210962 (lot AAA210962-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse Rictor Polyclonal Antibody | anti-Rictor antibody
Rabbit anti-Rictor Antibody, Affinity Purified
Gene Names
RICTOR; PIA; AVO3; hAVO3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Rictor, Antibody; Rabbit anti-Rictor Antibody, Affinity Purified; hAVO3; TORC2-specific protein AVO3; rapamycin-insensitive companion of mTOR; PIA; AVO3 homolog; AVO3; pianissimo; Rapamycin-insensitive companion of mTOR; RPTOR independent companion of MTOR complex 2; anti-Rictor antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Rictor antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1658 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse Rictor by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody AAA210962 (lot AAA210962-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse Rictor by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody AAA210962 (lot AAA210962-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human Rictor by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody AAA210962 (lot AAA210962-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Rictor by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody AAA210962 (lot AAA210962-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody AAA210962 (lot AAA210962-4) used for IP at 3 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot AAA210962-3) and rabbit anti-Rictor antibody For blotting immunoprecipitated Rictor, AAA210962 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody AAA210962 (lot AAA210962-4) used for IP at 3 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot AAA210962-3) and rabbit anti-Rictor antibody For blotting immunoprecipitated Rictor, AAA210962 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human Rictor by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor (Cat. No. AAA210962 Lot 4) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Rictor by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor (Cat. No. AAA210962 Lot 4) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Rictor antibody
Rictor is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6R327].
Product Categories/Family for anti-Rictor antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
195,011 Da
NCBI Official Full Name
rapamycin-insensitive companion of mTOR isoform 1
NCBI Official Synonym Full Names
RPTOR independent companion of MTOR, complex 2
NCBI Official Symbol
RICTOR
NCBI Official Synonym Symbols
PIA; AVO3; hAVO3
NCBI Protein Information
rapamycin-insensitive companion of mTOR; RICTOR; pianissimo; AVO3 homolog; TORC2-specific protein AVO3
UniProt Protein Name
Rapamycin-insensitive companion of mTOR
UniProt Gene Name
RICTOR
UniProt Synonym Gene Names
hAVO3
UniProt Entry Name
RICTR_HUMAN
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Product Notes
The Rictor rictor (Catalog #AAA210962) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Rictor Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rictor can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Rictor rictor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rictor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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